Anti-Caspase-7 antibody (ab52297)

Overview

  • Product nameAnti-Caspase-7 antibody
    See all Caspase-7 primary antibodies
  • Description
    Rabbit polyclonal to Caspase-7
  • Specificityab52297 detects endogenous levels of fragment of activated Caspase-7 resulting from cleavage adjacent to Asp198.
  • Tested applicationsSuitable for: IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-7.
    Database link: P55210

  • Positive control
    • Human lung carcinoma tissue.
  • General notes

    ab52297 is Cleaved at Asp198

Properties

Applications

Our Abpromise guarantee covers the use of ab52297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA 1/40000.

Target

  • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Overexpression promotes programmed cell death.
  • Tissue specificityHighly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
  • Sequence similaritiesBelongs to the peptidase C14A family.
  • Post-translational
    modifications
    Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptotic protease Mch-3 antibody
    • Apoptotic protease MCH3 antibody
    • CASP-7 antibody
    • CASP7 antibody
    • CASP7_HUMAN antibody
    • Caspase 7 antibody
    • Caspase 7 apoptosis related cysteine peptidase antibody
    • Caspase-7 subunit p11 antibody
    • Caspase7 antibody
    • CMH 1 antibody
    • CMH-1 antibody
    • CMH1 antibody
    • ICE LAP3 antibody
    • ICE-LAP3 antibody
    • ICE-like apoptotic protease 3 antibody
    • LICE2 antibody
    • MCH3 antibody
    see all

Anti-Caspase-7 antibody images

  • ab52297 at 1:50 dilution staining Caspase-7 in human lung carcinoma by Immunohistochemsitry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

    ab52297 at 1/50 dilution staining Caspase 7 in human lung carcinoma by Immunohistochemsitry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

References for Anti-Caspase-7 antibody (ab52297)

ab52297 has not yet been referenced specifically in any publications.

Product Wall

Merci de nous avoir contactés. Le laboratoire m'a informé que : L'anti-Caspase 7, référence ab52297, reconnaît les 2 formes, intacte et clivée. Nous aurions possibilité, si vous le souhaitez, d'ajouter &a...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Colorectal carcinoma)
Specification Colorectal carcinoma
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Mr. Philip Noble

Verified customer

Submitted Jun 10 2011

There is no antibody specific only for the activated form. As the antibody will recognize the same sequence despite whether it is in the pro-form or processed form. Pro-caspase antibodies are specific as the active version will not have the specific p...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"