Anti-Caspase-7 antibody (ab55427)


  • Product nameAnti-Caspase-7 antibody
    See all Caspase-7 primary antibodies
  • Description
    Rabbit polyclonal to Caspase-7
  • Specificityab55427 detects endogenous levels of fragment of activated Caspase-7 resulting from cleavage adjacent to Asp198.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-7.
    Database link: P55210

  • Positive control
    • Human lung carcinoma tissue and Jurkat cell extract, treated with Etoposide (25µM, 24hours).



Our Abpromise guarantee covers the use of ab55427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 34, 23 kDa (predicted molecular weight: 34 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.


  • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Overexpression promotes programmed cell death.
  • Tissue specificityHighly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
  • Sequence similaritiesBelongs to the peptidase C14A family.
  • Post-translational
    Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptotic protease Mch-3 antibody
    • Apoptotic protease MCH3 antibody
    • CASP-7 antibody
    • CASP7 antibody
    • CASP7_HUMAN antibody
    • Caspase 7 antibody
    • Caspase 7 apoptosis related cysteine peptidase antibody
    • Caspase-7 subunit p11 antibody
    • Caspase7 antibody
    • CMH 1 antibody
    • CMH-1 antibody
    • CMH1 antibody
    • ICE LAP3 antibody
    • ICE-LAP3 antibody
    • ICE-like apoptotic protease 3 antibody
    • LICE2 antibody
    • MCH3 antibody
    see all

Anti-Caspase-7 antibody images

  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab55427 (1:50 dilution), in the presence or absence of immunizing peptide.

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab55427 (1/50 dilution), in the presence or absence of immunizing peptide.
  • All lanes : Anti-Caspase-7 antibody (ab55427) at 1/500 dilution

    Lane 1 : Jurkat cell extract, treated with Etoposide (25µM, 24hours), without immunizing peptide
    Lane 2 : Jurkat cell extract, treated with Etoposide (25µM, 24hours), with immunizing peptide

    Predicted band size : 34 kDa
    Observed band size : 34 kDa
    Additional band at : 23 kDa is cleaved fragment.

References for Anti-Caspase-7 antibody (ab55427)

ab55427 has not yet been referenced specifically in any publications.

Product Wall

There is no antibody specific only for the activated form. As the antibody will recognize the same sequence despite whether it is in the pro-form or processed form. Pro-caspase antibodies are specific as the active version will not have the specific p...

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