Overview

  • Product name
    Anti-Caspase-7 antibody [E22]
    See all Caspase-7 primary antibodies
  • Description
    Rabbit monoclonal [E22] to Caspase-7
  • Specificity
    The antibody should recognize both pro-form and p20 cleaved-form. The antibody does not cross-react with other Caspase family members.
  • Tested applications
    Suitable for: Flow Cyt, WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caspase-7 aa 1-100 (N terminal).
    Database link: P55210

  • Positive control
    • WB: Jurkat & HeLa cell lysate. IHC-P: Human skin cancer tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
  • General notes

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-Caspase-7 antibody (HRP) [E22] (ab206039)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/250.
WB 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/100.
IP 1/10.

Target

  • Function
    Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Overexpression promotes programmed cell death.
  • Tissue specificity
    Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
  • Sequence similarities
    Belongs to the peptidase C14A family.
  • Post-translational
    modifications
    Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptotic protease Mch-3 antibody
    • Apoptotic protease MCH3 antibody
    • CASP-7 antibody
    • CASP7 antibody
    • CASP7_HUMAN antibody
    • Caspase 7 antibody
    • Caspase 7 apoptosis related cysteine peptidase antibody
    • Caspase-7 subunit p11 antibody
    • Caspase7 antibody
    • CMH 1 antibody
    • CMH-1 antibody
    • CMH1 antibody
    • ICE LAP3 antibody
    • ICE-LAP3 antibody
    • ICE-like apoptotic protease 3 antibody
    • LICE2 antibody
    • MCH3 antibody
    see all

Images



  • Predicted band size : 34 kDa

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: Wild type HAP1 + Staurosporin whole cell lysate (20 µg)
    Lane 3: CASP7 knockout HAP1 whole cell lysate (20 µg)
    Lane 4: CASP7 + Staurosporin knockout HAP1 whole cell lysate (20 µg)
    Lane 5: HeLa whole cell lysate (20 µg)
    Lane 6: HeLa + Staurosporin whole cell lysate (20 µg)

    Lanes 1 - 6: Merged signal (red and green). Green - ab32522 observed at 38 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab32522 was shown to specifically react with HAP1 + Staurosporin when HAP1 + Staurosporin knockout samples were used. Wild-type and HAP1 + Staurosporin knockout samples were subjected to SDS-PAGE. Ab32522 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Anti-Caspase-7 antibody [E22] (ab32522) at 1/1000 dilution + Jurkat cell lysate

    Predicted band size : 34 kDa
    Observed band size : 34 kDa
  • Immunohistochemical analysis of paraffin embedded human skin cancer tissue using ab32522 at 1:50 dilution.

  • Immunofluorescent staining of HeLa cells using ab32522 at 1:100 dilution.

  • Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Caspase-7 (red) with ab32522 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

References

This product has been referenced in:
  • Chaudhary S  et al. Overexpression of caspase 7 is ERa dependent to affect proliferation and cell growth in breast cancer cells by targeting p21(Cip). Oncogenesis 5:e219 (2016). WB, IF . Read more (PubMed: 27089142) »
  • Wang X  et al. Synergistic control of sex hormones by 17ß-HSD type 7: a novel target for estrogen-dependent breast cancer. J Mol Cell Biol 7:568-79 (2015). Read more (PubMed: 25966904) »

See all 4 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Human Cell lysate - whole cell (MCF7)
Specification
MCF7
Total protein in input
100 µg
Username

Abcam user community

Verified customer

Submitted Jul 05 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (8)
Sample
Human Cell lysate - whole cell (MCF7)
Specification
MCF7
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Jul 04 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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