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Abcam's Caspase 7 Immunoassay Kit (Fluorometric) provides an effective immunosorbent enzyme assay for the specific, quantitative detection of Caspase 7 activity in microtiter plates. The assay utilizes Caspase 7 polyclonal antibody to capture activated Caspase 7 from cell lysates. Substrate DEVD-AFC is then added that is cleaved proportionally to the amount of activated Caspase 7 in the cell lysate. The cleavage generates free AFC which is then analyzed fluorometrically (Ex./Em. = 400/505 nm) using a fluorescence plate reader. The assay ensures absolute specific detection of Caspase 7. Other known caspases and non-specific proteases are not detected.
|Adhesive Plate Cover||2 units|
|Anti Caspase 7 anitbody||Blue||1 x 0.5ml|
|Blocking Buffer||WM||1 x 15ml|
|Caspase 7 Positive Control||Red||10 units|
|Cell Lysis Buffer||WM||1 x 25ml|
|Coating Buffer||NM||1 x 10ml|
|DEVD-AFC Substrate||Brown||1 x 500µl|
|DTT||1 x 400µl|
|Incubation Buffer||NM||1 x 100ml|
|Microtiter plate||12 x 8 units|
Our Abpromise guarantee covers the use of ab157404 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"