Caspase-7 Immunoassay Activity Kit (Fluorometric) (ab157404)

Overview

  • Product name
    Caspase-7 Immunoassay Activity Kit (Fluorometric)
  • Sample type
    Cell Lysate, Tissue Lysate
  • Assay type
    Quantitative
  • Assay time
    4h 00m
  • Species reactivity

    Predicted to work with: Mammal
  • Product overview

    Abcam's Caspase 7 Immunoassay Kit (Fluorometric) provides an effective immunosorbent enzyme assay for the specific, quantitative detection of Caspase 7 activity in microtiter plates. The assay utilizes Caspase 7 polyclonal antibody to capture activated Caspase 7 from cell lysates. Substrate DEVD-AFC is then added that is cleaved proportionally to the amount of activated Caspase 7 in the cell lysate. The cleavage generates free AFC which is then analyzed fluorometrically (Ex./Em. = 400/505 nm) using a fluorescence plate reader. The assay ensures absolute specific detection of Caspase 7. Other known caspases and non-specific proteases are not detected.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab157404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Protocols

References

ab157404 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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