Overview

  • Product nameCaspase 8 (active) Red Staining Kit
    See all Caspase 8 kits
  • Tests
    100 x 1 assay
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeEnzyme activity
  • Assay time
    2h 00m
  • Product overview

    Abcam's Caspase 8 (active) Red Staining Kit provides a convenient means for detecting activated caspase 8 in living cells. The assay utilizes the caspase 8 inhibitor IETD-FMK conjugated to sulfo-rhodamine (Red-IETD-FMK) as the fluorescent in situ marker. Red-IETD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase 8 in apoptotic cells.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Activation of caspases plays a central role in apoptosis.

  • Tested applicationsSuitable for: FM, Functional Studies, Flow Cytmore details

Properties

  • RelevanceCaspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.
  • Cellular localizationCytoplasmic
  • Alternative names
    • Apoptotic cysteine protease
    • CAP4
    • CASP8
    • Caspase 8
    • CED 3
    • FLICE
    • MACH
    • MACH5
    see all

Applications

Our Abpromise guarantee covers the use of ab65618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.
Functional Studies Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.

Protocols

References for Caspase 8 (active) Red Staining Kit (ab65618)

ab65618 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"