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Synthetic peptide corresponding to Human Caspase-8 (C terminal). Synthetic peptide (Human) - a 16 amino acid peptide from near the carboxy-terminus of human Caspase-8 isoform A.
Database link: Q14790
Our Abpromise guarantee covers the use of ab25901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|IHC-P||Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC||Use a concentration of 2 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).|
Immunohistochemistry of Caspase-8 in human spleen tissue with Caspase-8 antibody at 5 µg/ml.
ab25901 at 2ug/ml staining Caspase-8 in Jurkat cells by ICC/IF
IHC image of Caspase-8 staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25901, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.