Anti-Caspase-8 antibody, prediluted (ab15552)

Overview

  • Product nameAnti-Caspase-8 antibody, prediluted
    See all Caspase-8 primary antibodies
  • Description
    Rabbit polyclonal to Caspase-8, prediluted
  • SpecificityThis antibody detects both full length Caspase-8 and the processed forms(42/44kDa,25kDa and 14kDa).
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human, Pig
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-8. Synthetic peptide corresponding to N-terminus of human caspase-8 protein.
    Database link: Q14790

  • Positive control
    • Jurkat cells or Stomach

Applications

Our Abpromise guarantee covers the use of ab15552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. See Abreview.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionMost upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-
    -AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
  • Tissue specificityIsoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.
  • Involvement in diseaseDefects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. CASP8D is a disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization.
  • Sequence similaritiesBelongs to the peptidase C14A family.
    Contains 2 DED (death effector) domains.
  • DomainIsoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex.
  • Post-translational
    modifications
    Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALPS2B antibody
    • Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein antibody
    • Apoptotic cysteine protease antibody
    • Apoptotic protease Mch-5 antibody
    • Apoptotic protease Mch5 antibody
    • CAP4 antibody
    • CASP-8 antibody
    • CASP8 antibody
    • CASP8_HUMAN antibody
    • Caspase 8 antibody
    • Caspase 8 apoptosis related cysteine peptidase antibody
    • Caspase-8 subunit p10 antibody
    • CED 3 antibody
    • FADD Like ICE antibody
    • FADD-homologous ICE/CED-3-like protease antibody
    • FADD-like ICE antibody
    • FLICE antibody
    • FLJ17672 antibody
    • ICE-like apoptotic protease 5 antibody
    • MACH alpha 1/2/3 protein antibody
    • MACH antibody
    • MACH beta 1/2/3/4 protein antibody
    • MCH5 antibody
    • MGC78473 antibody
    • MORT1 associated ced 3 homolog antibody
    • MORT1-associated CED-3 homolog antibody
    • OTTHUMP00000163717 antibody
    • OTTHUMP00000163720 antibody
    • OTTHUMP00000163724 antibody
    • OTTHUMP00000163725 antibody
    • OTTHUMP00000165062 antibody
    • OTTHUMP00000165063 antibody
    • OTTHUMP00000165064 antibody
    • OTTHUMP00000206552 antibody
    • OTTHUMP00000206582 antibody
    see all

Anti-Caspase-8 antibody, prediluted images

  • Human stomach stained with anti-caspase-8. For antigen retrieval, boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

References for Anti-Caspase-8 antibody, prediluted (ab15552)

This product has been referenced in:
  • Qiu Q  et al. Time-Course of the Effects of QSYQ in Promoting Heart Function in Ameroid Constrictor-Induced Myocardial Ischemia Pigs. Evid Based Complement Alternat Med 2014:571076 (2014). WB ; Pig . Read more (PubMed: 24817898) »
  • Hilbe M  et al. Apoptosis in Bovine Viral Diarrhea Virus (BVDV)-Induced Mucosal Disease Lesions: A Histological, Immunohistological, and Virological Investigation. Vet Pathol : (2012). IHC-P ; Cow . Read more (PubMed: 22700847) »

See all 3 Publications for this product

Product Wall

Thank you for your reply and the additional information.

Thank you for sending your data.From the images,I am not sure right nowwhich images refer to caspase 8 staining and which refer to MMP2 staining. Are these images for the skin tumor se...

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Thank you for your reply and the additional information.

I do have a few more questions as well as suggestions:

1) Are the skin sections from human or any other species? Are you also using cardiomyocytes? if so, which species are t...

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Thank you for contacting us.

I am sorry to hear that these 2 antibodies are not working as expected.

Could you please confirm that you are refering to the antibodies ab15552 and ab54401?
I assume you were using them in paraffi...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Pig Cell lysate - whole cell (Porcine aortic endothelial cells)
Loading amount 2 µg
Specification Porcine aortic endothelial cells
Gel Running Conditions Reduced Denaturing (15%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

Submitted May 16 2008

This antibody detects both full length and the processed forms(42/44kDa,25kDa and 14kDa). Please contact us again if you have any additional questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"