Overview

  • Product name
    Caspase 8 Assay Kit (Fluorometric)
    See all Caspase 8 kits
  • Sample type
    Tissue Extracts, Cell Lysate
  • Assay type
    Enzyme activity
  • Assay time
    2h 00m
  • Product overview

    Abcam's Caspase 8 Assay Kit (Fluorometric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on detection of cleavage of substrate IETD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). IETD-AFC emits blue light (?max = 400 nm); upon cleavage of the substrate by caspase 8 or related caspases, free AFC emits a yellow-green fluorescence (?max = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab39534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Protocols

References

This product has been referenced in:
  • Buvall L  et al. Orellanine specifically targets renal clear cell carcinoma. Oncotarget 8:91085-91098 (2017). Functional Studies . Read more (PubMed: 29207627) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab39534.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up