Overview

  • Product nameCaspase-8 Human ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Serum 8 6.7%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum, Cell culture extracts, Suspension cells
  • Assay typeSandwich (quantitative)
  • Sensitivity
    0.1 ng/ml
  • Range
    0.16 ng/ml - 10 ng/ml
  • Recovery

    89.5 %

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Caspase-8 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Caspase-8 concentrations in cell culture supernatant, cell lysate and serum.

    Caspase-8 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with a Caspase-8 detection antibody and the microplate is then incubated at room temperature. Following washing a HRP conjugated anti-rabbit-IgG antibody is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Caspase-8 amount of sample captured in plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Assay Buffer Concentrate 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 4 units
    Anti-rabbit-IgG HRP conjugated antibody 1 x 10µl
    Blue-Dye 1 x 400µl
    Green-Dye 1 x 400µl
    Human Caspase-8 Standard 2 vials
    Lysis Buffer (10x) 1 x 15ml
    Microplate coated with monoclonal antibody to Human Caspase-8 (12 x 8 wells) 1 unit
    Polyclonal anti-Human Caspase-8 Detection Antibody (rabbit) 1 x 100µl
    Red-Dye 1 x 400µl
    Sample Diluent 1 x 12ml
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    TMB Substrate Solution 1 x 15ml
  • Research Areas
  • FunctionMost upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-
    -AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
  • Tissue specificityIsoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.
  • Involvement in diseaseDefects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. CASP8D is a disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization.
  • Sequence similaritiesBelongs to the peptidase C14A family.
    Contains 2 DED (death effector) domains.
  • DomainIsoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex.
  • Post-translational
    modifications
    Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Alternative names
    • Apoptotic cysteine protease
    • Apoptotic protease Mch-5
    • CAP4
    • CASP-8
    • CASP8
    • CASP8_HUMAN
    • Caspase-8 subunit p10
    • FADD-homologous ICE/CED-3-like protease
    • FADD-like ICE
    • FLICE
    • ICE-like apoptotic protease 5
    • MACH
    • MORT1-associated CED-3 homolog
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Caspase-8 Human ELISA Kit images

  • Representative Standard Curve using ab119507.

Protocols

References for Caspase-8 Human ELISA Kit (ab119507)

ab119507 has not yet been referenced specifically in any publications.

Product Wall



The lysis buffer in kits ab119507 and ab119521 are identical, so you can analyze lysates prepared for ab119507 with ab119521.
For ab119521, the lysis buffer allows for lysis of the cellular membrane but not other compartments with in the...

Read More


The lysis buffers in both kits (ab119507 and ab119508) are the same and thus interchangeable.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"