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Abcam’s Caspase-8 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Caspase-8 concentrations in cell culture supernatant, cell lysate and serum.
Caspase-8 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with a Caspase-8 detection antibody and the microplate is then incubated at room temperature. Following washing a HRP conjugated anti-rabbit-IgG antibody is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Caspase-8 amount of sample captured in plate.
|Components||1 x 96 tests|
|20X Assay Buffer Concentrate||1 x 5ml|
|20X Wash Buffer Concentrate||1 x 50ml|
|Adhesive Films||4 units|
|Anti-rabbit-IgG HRP conjugated antibody||1 x 10µl|
|Blue-Dye||1 x 400µl|
|Green-Dye||1 x 400µl|
|Human Caspase-8 Standard||2 vials|
|Lysis Buffer (10x)||1 x 15ml|
|Microplate coated with monoclonal antibody to Human Caspase-8 (12 x 8 wells)||1 unit|
|Polyclonal anti-Human Caspase-8 Detection Antibody (rabbit)||1 x 100µl|
|Red-Dye||1 x 400µl|
|Sample Diluent||1 x 12ml|
|Stop Solution (1M Phosphoric acid)||1 x 15ml|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab119507 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab119507 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"