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Abcam's Caspase 9 (active) Red Staining Kit provides a convenient means for detecting activated caspase 9 in living cells. The assay utilizes the caspase 9 inhibitor LEHD-FMK conjugated to sulfo-rhodamine (Red-LEHD-FMK) as the fluorescent in situ marker. Red-LEHD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase 9 in apoptotic cells.
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Activation of caspases plays a central role in apoptosis.
|Red-LEHD-FMK||1 x 100µl|
|Wash Buffer||2 x 100ml|
|Z-VAD-FMK||1 x 10µl|
Our Abpromise guarantee covers the use of ab65619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|FM||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent dilution.|
|Flow Cyt||Use at an assay dependent dilution.|
ab65619 has not yet been referenced specifically in any publications.