Overview

  • Product nameAnti-Caspase-9 antibody
    See all Caspase-9 primary antibodies
  • Description
    Rabbit polyclonal to Caspase-9
  • SpecificityNo cross reactivity with other members of the caspase family. This antibody does not recognise the cleaved form of Caspase-9.
  • Tested applicationsSuitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-9 aa 41-56.
    Sequence:

    EDIQRAGSGSRRDQAR


    Database link: P55211
    (Peptide available as ab7885)

  • Positive control
    • HeLa whole cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab2013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB 1/1000 - 1/2000. Predicted molecular weight: 46 kDa.Can be blocked with Human Caspase-9 peptide (ab7885).

Target

  • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
    Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
  • Tissue specificityUbiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
  • Sequence similaritiesBelongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Developmental stageExpressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
  • Post-translational
    modifications
    Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
  • Information by UniProt
  • Database links
  • Alternative names
    • APAF-3 antibody
    • APAF3 antibody
    • Apoptosis related cysteine peptidase antibody
    • Apoptotic protease Mch-6 antibody
    • Apoptotic protease-activating factor 3 antibody
    • CASP-9 antibody
    • CASP9 antibody
    • CASP9_HUMAN antibody
    • Caspase 9 apoptosis related cysteine peptidase antibody
    • Caspase 9 Dominant Negative antibody
    • Caspase 9c antibody
    • Caspase-9 antibody
    • Caspase-9 subunit p10 antibody
    • ICE LAP6 antibody
    • ICE like apoptotic protease 6 antibody
    • ICE-LAP6 antibody
    • ICE-like apoptotic protease 6 antibody
    • MCH6 antibody
    • PPP1R56 antibody
    • protein phosphatase 1, regulatory subunit 56 antibody
    • RNCASP9 antibody
    see all

Anti-Caspase-9 antibody images

  • Anti-Caspase-9 antibody (ab2013) at 1/1000 dilution + HeLa whole cell lysate

    Predicted band size : 46 kDa
    Observed band size : 46 kDa

References for Anti-Caspase-9 antibody (ab2013)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Western blot
Sample Pig Cell lysate - other (Lion, muscle tissue)
Gel Running Conditions Reduced Denaturing (8-16%)
Loading amount 150 µg
Specification Lion, muscle tissue
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Ms. Mayra González

Verified customer

Submitted Jul 14 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (stomach cancer)
Loading amount 25 µg
Specification stomach cancer
Treatment 10uM WIN 55,212-2 for 24hrs
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 27 2013

Thank you for your enquiry. Antibody ab2013 will recognize the recombinant CASP9 protein ab52203. Based on the units of ab52203 per vial received, you will receive about 62ug recombinant caspase 9 protein in a vial of ab52203. I hope this is helpful. P...

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Thank you for your enquiry. Further to our telephone conversation this morning I have updated our datasheet regarding the specificity of this antibody for the uncleaved form of Caspase 9. I look forward to reading your Abreview. I have arranged...

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Thank you for your email. I have performed a search of our catalogue and I can tell you that ab28142 and ab2013 are your best antibodies for the detection of cleaved and uncleaved caspase 8 and 9 by western blotting. The notes of each antibody are a...

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No, to our knowledge, this anitbody has only been tested in WB.

This antibody was made with a peptide located at amino acids 41-56 of human caspase-9. The peptide is located in the cleaved and activated fragment (35 or 37 kDa). Therefore, it should recognize the precursor and activated form although we have not t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"