Overview

  • Product nameAnti-Caspase-9 antibody
    See all Caspase-9 primary antibodies
  • Description
    Rabbit polyclonal to Caspase-9
  • SpecificityNo cross-reactivity to other members of the caspase family.
  • Tested applicationsSuitable for: IP, ICC/IF, ICC, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-9 aa 299-318.
    Sequence:

    ASTSPEDESPGSNPEPDATP


    Database link: P55211
    (Peptide available as ab7886)

  • Positive control
    • HeLa whole cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab2014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ICC/IF Use a concentration of 20 µg/ml.
ICC Use a concentration of 2 µg/ml.
WB 1/1000 - 1/2000. Can be blocked with Human Caspase-9 peptide (ab7886).
IHC-P Use at an assay dependent concentration.

Target

  • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
    Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
  • Tissue specificityUbiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
  • Sequence similaritiesBelongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Developmental stageExpressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
  • Post-translational
    modifications
    Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
  • Information by UniProt
  • Database links
  • Alternative names
    • APAF-3 antibody
    • APAF3 antibody
    • Apoptosis related cysteine peptidase antibody
    • Apoptotic protease Mch-6 antibody
    • Apoptotic protease-activating factor 3 antibody
    • CASP-9 antibody
    • CASP9 antibody
    • CASP9_HUMAN antibody
    • Caspase 9 apoptosis related cysteine peptidase antibody
    • Caspase 9 Dominant Negative antibody
    • Caspase 9c antibody
    • Caspase-9 antibody
    • Caspase-9 subunit p10 antibody
    • ICE LAP6 antibody
    • ICE like apoptotic protease 6 antibody
    • ICE-LAP6 antibody
    • ICE-like apoptotic protease 6 antibody
    • MCH6 antibody
    • PPP1R56 antibody
    • protein phosphatase 1, regulatory subunit 56 antibody
    • RNCASP9 antibody
    see all

Anti-Caspase-9 antibody images

  • Anti-Caspase-9 antibody (ab2014) at 1/1000 dilution + HeLa whole cell lysate.

    Observed band size : 46 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence of Caspase-9 in K562 cells using ab2014 at 20 ug/ml.

  • ab2014 at 2µg/ml staining Caspase-9 in K562 cells by Immunocytochemistry.

References for Anti-Caspase-9 antibody (ab2014)

This product has been referenced in:
  • Minutoli L  et al. Mitogen-activated protein kinase 3/mitogen-activated protein kinase 1 activates apoptosis during testicular ischemia-reperfusion injury in a nuclear factor-kappaB-independent manner. Eur J Pharmacol 604:27-35 (2009). WB ; Mouse . Read more (PubMed: 19135439) »
  • Quintarelli C  et al. Co-expression of cytokine and suicide genes to enhance the activity and safety of tumor-specific cytotoxic T lymphocytes. Blood 110:2793-802 (2007). WB . Read more (PubMed: 17638856) »

See all 3 Publications for this product

Product Wall

Merci de nous avoir contactés.

Nous sommes désolés d'apprendre que l'anti-caspase-9 ab2014 que vous avez reçu n'a pas fonctionné. Je me permets de vous transférer le numéro de commande de rempla...

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La référence ab2014 n'a pas encore été testée chez le rat, c'est pour cela que j'ai préféré comparer les séquences.

Nous serions ravis si vous pouviez nous communiquer vos r&eacut...

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Merci pour votre réponse.

L'anticorps ab63488 est en effet garanti pour fonctionner en WB et IHC-P. Je suis d'accord avec vous, l'image du blotn'est pas très attractive.

Une autre référence qui a retenu m...

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It should detect the cleaved and activated fragment (35 or 37 kDa) as well as the whole protein of caspase-9.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"