Overview

  • Product name
  • Description
    Rabbit polyclonal to Caspase-9
  • Specificity
    ab25758 detects 45 kDa procaspase-9, 35/37 kDa cleaved large fragment and the 25 kDa fully cleaved caspase-9. The antibody does not detect any other caspases.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to Mouse Caspase-9.
    (Peptide available as ab38268)

Properties

Applications

Our Abpromise guarantee covers the use of ab25758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 46 kDa).Can be blocked with Mouse Caspase-9 peptide (ab38268).

Target

  • Function
    Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
    Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
  • Tissue specificity
    Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
  • Sequence similarities
    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Developmental stage
    Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
  • Post-translational
    modifications
    Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
  • Information by UniProt
  • Database links
  • Alternative names
    • APAF-3 antibody
    • APAF3 antibody
    • Apoptosis related cysteine peptidase antibody
    • Apoptotic protease Mch-6 antibody
    • Apoptotic protease-activating factor 3 antibody
    • CASP-9 antibody
    • CASP9 antibody
    • CASP9_HUMAN antibody
    • Caspase 9 apoptosis related cysteine peptidase antibody
    • Caspase 9 Dominant Negative antibody
    • Caspase 9c antibody
    • Caspase-9 antibody
    • Caspase-9 subunit p10 antibody
    • ICE LAP6 antibody
    • ICE like apoptotic protease 6 antibody
    • ICE-LAP6 antibody
    • ICE-like apoptotic protease 6 antibody
    • MCH6 antibody
    • PPP1R56 antibody
    • protein phosphatase 1, regulatory subunit 56 antibody
    • RNCASP9 antibody
    see all

Images

  • Anti-Caspase-9 antibody (ab25758) + mouse intestine tissue lysate

    Predicted band size : 46 kDa
    The antibody detects 45 kDa procaspase 9, 35/37 kDa cleaved immediate fragment and the 17-25 kDa fully cleaved caspase 9.

References

This product has been referenced in:
  • Liu Y  et al. Hepatoprotective Effects of Antrodia cinnamomea: The Modulation of Oxidative Stress Signaling in a Mouse Model of Alcohol-Induced Acute Liver Injury. Oxid Med Cell Longev 2017:7841823 (2017). Read more (PubMed: 28337253) »
  • Lin Z  et al. Suppression of GLI sensitizes medulloblastoma cells to mitochondria-mediated apoptosis. J Cancer Res Clin Oncol 142:2469-2478 (2016). Read more (PubMed: 27601167) »

See all 8 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

As this is an exceptional free of charge replacement, I will process the order. Could you please confirm that you would like a free of charge replacement with ab25758 and not ab133520 (rabbit monoclonal I recomme...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Human breast tumour)
Specification
Human breast tumour
Treatment
2mg/kg doxorubicin and 0.1mg/kg zoledronic acid
Gel Running Conditions
Reduced Non-Denaturing (Native) (10 % polyacrylamide gel)
Blocking step
(agent) for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Dr. Penelope Ottewell

Verified customer

Submitted Jul 04 2008

I can confirm that the antibody does not detect any other caspases. Please do not hesitate to contact us if you require further information,

Thank you for your enquiry. Further to our telephone conversation this morning I have updated our datasheet regarding the specificity of this antibody for the uncleaved form of Caspase 9. I look forward to reading your Abreview. I have arranged...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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