The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 20520761
Use at an assay dependent concentration. PubMed: 21765948
Use at an assay dependent concentration.
FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
Tissue specificityUbiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
Sequence similaritiesBelongs to the peptidase C14A family. Contains 1 CARD domain.
Developmental stageExpressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
Post-translational modificationsCleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
ab52298 at 1/50 dilution staining Caspase 9 in human lung
carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
Immunocytochemistry/ Immunofluorescence - Anti-Caspase-9 antibody (ab52298)Image from Polo ML et al, PLoS One. 2010 May 26;5(5):e10786, Fig 5.
ab52298 staining Caspase 9 - Cleaved Asp353 in mouse primary C4-HI cancer cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in 10% formalin for 20 minutes at room temperature and incubated overnight at 4°C with the primary antibody. The corresponding secondary FITC-conjugated antibody was used at 1/100 dilution and incubated for 1 hour at room temperature. Nuclei were stained red with propidium iodide.
Immunohistochemistry (Frozen sections) - Anti-Caspase-9 antibody (ab52298)Image from Kaur J et al., PLoS One. 2011;6(7):e22181. Epub 2011 Jul 12. Fig S3.; doi:10.1371/journal.pone.0022181July 12, 2011, PLoS ONE 6(7): e22181.
Immunohistochemical analysis of frozen retina tissue, taken from wild-type (top) or S334ter rhodopsin transgenic rats (bottom).
Caspase 9 - Cleaved Asp353 was stained using ab52298 at 1/100 dilution. An AlexaFluor® 488-conjugated IgG was used as the secondary antibody.
Lam W et al. PHY906(KD018), an adjuvant based on a 1800-year-old Chinese medicine, enhanced the anti-tumor activity of Sorafenib by changing the tumor microenvironment. Sci Rep5:9384 (2015).
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