• Product nameAnti-Caspase-9 antibody
    See all Caspase-9 primary antibodies
  • Description
    Rabbit polyclonal to Caspase-9
  • Specificityab63342 detects endogenous levels of total Caspase-9 protein.
  • Tested applicationsSuitable for: ELISA, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-9.
    Database link: P55211

  • Positive control
    • HeLa cells, treated with EGF, HeLa cells



Our Abpromise guarantee covers the use of ab63342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.
WB Use at an assay dependent concentration. Detects a band of approximately 36 kDa (predicted molecular weight: 46 kDa).
IHC-P 1/50 - 1/100.


  • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
    Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
  • Tissue specificityUbiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
  • Sequence similaritiesBelongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Developmental stageExpressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
  • Post-translational
    Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
  • Information by UniProt
  • Database links
  • Alternative names
    • APAF-3 antibody
    • APAF3 antibody
    • Apoptosis related cysteine peptidase antibody
    • Apoptotic protease Mch-6 antibody
    • Apoptotic protease-activating factor 3 antibody
    • CASP-9 antibody
    • CASP9 antibody
    • CASP9_HUMAN antibody
    • Caspase 9 apoptosis related cysteine peptidase antibody
    • Caspase 9 Dominant Negative antibody
    • Caspase 9c antibody
    • Caspase-9 antibody
    • Caspase-9 subunit p10 antibody
    • ICE LAP6 antibody
    • ICE like apoptotic protease 6 antibody
    • ICE-LAP6 antibody
    • ICE-like apoptotic protease 6 antibody
    • MCH6 antibody
    • PPP1R56 antibody
    • protein phosphatase 1, regulatory subunit 56 antibody
    • RNCASP9 antibody
    see all

Anti-Caspase-9 antibody images

  • All lanes : Anti-Caspase-9 antibody (ab63342)

    Lane 1 : extracts from HeLa cells, treated with EGF (200ng/ml, 30mins), without immunising peptide
    Lane 2 : extracts from HeLa cells, treated with EGF (200ng/ml, 30mins), with immunising peptide

    Predicted band size : 46 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
    The amount of cell extracts loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.
  • Immunofluorescence analysis of Caspase-9 expression in HeLa cells, using 1/50-1/100 ab63342. Left: untreated sample. Right: sample treated with immunising peptide.

  • Ab63342 staining Human tonsil. Staining is localised to the cytoplasm.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-Caspase-9 antibody (ab63342)

This product has been referenced in:
  • da Silva Santos C  et al. Proteome profiling of human cutaneous leishmaniasis lesion. J Invest Dermatol 135:400-10 (2015). IHC ; Human . Read more (PubMed: 25207817) »

See 1 Publication for this product

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