Overview

  • Product nameAnti-Caspase 9 antibody
    See all Caspase-9 primary antibodies
  • Description
    Rabbit polyclonal to Caspase 9
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-9.
    Database link: P55211

  • Positive control
    • IHC-P: Human lung carcinoma tissue. WB: Extracts from NIH/3T3 cells treated with TNF alpha (20ng/ml, 30min). ICC/IF: HuvEc cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab63488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 46 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.

Target

  • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
    Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
  • Tissue specificityUbiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
  • Sequence similaritiesBelongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Developmental stageExpressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
  • Post-translational
    modifications
    Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
  • Information by UniProt
  • Database links
  • Alternative names
    • APAF-3 antibody
    • APAF3 antibody
    • Apoptosis related cysteine peptidase antibody
    • Apoptotic protease Mch-6 antibody
    • Apoptotic protease-activating factor 3 antibody
    • CASP-9 antibody
    • CASP9 antibody
    • CASP9_HUMAN antibody
    • Caspase 9 apoptosis related cysteine peptidase antibody
    • Caspase 9 Dominant Negative antibody
    • Caspase 9c antibody
    • Caspase-9 antibody
    • Caspase-9 subunit p10 antibody
    • ICE LAP6 antibody
    • ICE like apoptotic protease 6 antibody
    • ICE-LAP6 antibody
    • ICE-like apoptotic protease 6 antibody
    • MCH6 antibody
    • PPP1R56 antibody
    • protein phosphatase 1, regulatory subunit 56 antibody
    • RNCASP9 antibody
    see all

Anti-Caspase 9 antibody images

  • This image shows HuvEc cells stained with ab63488 at 1/500 dilution; with and without the immunising peptide.
  • This image shows human lung carcinoma tissue stained with ab63488 at 1/50 dilution; with and without the immunising peptide.
  • All lanes : Anti-Caspase 9 antibody (ab63488) at 1/500 dilution

    Lane 1 : Extracts from NIH/3T3 cells (5-30 µg total protein) treated with TNF-alpha (20ng/ml, 30min)
    Lane 2 : Extracts from NIH/3T3 cells (5-30 µg total protein) treated with TNF-alpha (20ng/ml, 30min) and the immunising peptide (5-10µg).


    Predicted band size : 46 kDa
    Observed band size : 46 kDa

References for Anti-Caspase 9 antibody (ab63488)

This product has been referenced in:
  • Fani S  et al. Monobenzyltin Complex C1 Induces Apoptosis in MCF-7 Breast Cancer Cells through the Intrinsic Signaling Pathway and through the Targeting of MCF-7-Derived Breast Cancer Stem Cells via the Wnt/ß-Catenin Signaling Pathway. PLoS One 11:e0160836 (2016). Human . Read more (PubMed: 27529753) »
  • Stacchiotti A  et al. Mitochondrial and metabolic dysfunction in renal convoluted tubules of obese mice: protective role of melatonin. PLoS One 9:e111141 (2014). ICC/IF ; Mouse . Read more (PubMed: 25347680) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry and your interest in our products.

I can confirm that ab63488 has not been specifically tested for cross-reactivity with rat, it is only predicted to work in this species based on sequence homology.

Howeve...

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Thank you for contacting us. I have checked the antibodies we have in our catalog to Caspase 9, and none of them are likely to show any cross-reactivity with DRONC. The full length proteins are only 24% homologous, so it is unlikely that you would be a...

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Thank you for contacting us.



Ab63488 Anti-Caspase 9 antibody is raised against an immunogen corresponding to aa100-150 of human Caspase 9 with ACC# P55211 (http://www.uniprot.org/uniprot/P55211). This antibody binds to N-terminal...

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Merci pour votre réponse.

L'anticorps ab63488 est en effet garanti pour fonctionner en WB et IHC-P. Je suis d'accord avec vous, l'image du blotn'est pas très attractive.

Une autre référence qui a retenu m...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"