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Abcam's Caspase 9 Assay Kit (Colorimetric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (p-NA) after cleavage from the labeled substrate LEHD-p-NA. The p-NA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
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Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.
|2X Reaction Buffer||4 x 2ml|
|Cell Lysis Buffer||1 x 100ml|
|Dilution Buffer||1 x 100ml|
|DTT||1 x 400µl|
|LEHD-pNA (4mM)||1 x 500µl|
Our Abpromise guarantee covers the use of ab65608 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|