Anti-Caspase-9 (phospho S144) antibody (ab61004)

Overview

  • Product nameAnti-Caspase-9 (phospho S144) antibody
    See all Caspase-9 primary antibodies
  • Description
    Rabbit polyclonal to Caspase-9 (phospho S144)
  • SpecificityDetects endogenous levels of Caspase-9 only when phosphorylated at serine 144.
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-9.
    Database link: P55211

  • Positive control
    • WB: Extracts from K562 cells. IHC-P: Human breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab61004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 46 kDa).

Target

  • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
    Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
  • Tissue specificityUbiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
  • Sequence similaritiesBelongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Developmental stageExpressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
  • Post-translational
    modifications
    Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
  • Information by UniProt
  • Database links
  • Alternative names
    • APAF-3 antibody
    • APAF3 antibody
    • Apoptosis related cysteine peptidase antibody
    • Apoptotic protease Mch-6 antibody
    • Apoptotic protease-activating factor 3 antibody
    • CASP-9 antibody
    • CASP9 antibody
    • CASP9_HUMAN antibody
    • Caspase 9 apoptosis related cysteine peptidase antibody
    • Caspase 9 Dominant Negative antibody
    • Caspase 9c antibody
    • Caspase-9 antibody
    • Caspase-9 subunit p10 antibody
    • ICE LAP6 antibody
    • ICE like apoptotic protease 6 antibody
    • ICE-LAP6 antibody
    • ICE-like apoptotic protease 6 antibody
    • MCH6 antibody
    • PPP1R56 antibody
    • protein phosphatase 1, regulatory subunit 56 antibody
    • RNCASP9 antibody
    see all

Anti-Caspase-9 (phospho S144) antibody images

  • All lanes : Anti-Caspase-9 (phospho S144) antibody (ab61004) at 1/500 dilution

    Lane 1 : extracts from K562 cells
    Lane 2 : extracts from K562 cells with immunizing phosphopeptide


    Predicted band size : 46 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • ab61004, at a 1/50 dilution, staining phosphoriylated Caspase-9 in human breast carcinoma tissue sections by Immunohistochemistry in the absence (left) or presence (right) of the immunizing phosphopeptide.

References for Anti-Caspase-9 (phospho S144) antibody (ab61004)

ab61004 has not yet been referenced specifically in any publications.

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