Overview

  • Product nameAnti-Caspase-9 (phospho S196) antibody
    See all Caspase-9 primary antibodies
  • Description
    Rabbit polyclonal to Caspase-9 (phospho S196)
  • Specificityab61789 detects Caspase-9 only when phosphorylated at serine 196.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-9. Synthesized phosphopeptide derived from human Caspase 9 around the phosphorylation site of serine 196 (F-S-SP-L-H).
    Database link: P55211

  • Positive control
    • Extracts from 293 cells, treated with Calyculin (50nM, 30mins)

Properties

Applications

Our Abpromise guarantee covers the use of ab61789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
ELISA 1/20000.

Target

  • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
    Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
  • Tissue specificityUbiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
  • Sequence similaritiesBelongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Developmental stageExpressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
  • Post-translational
    modifications
    Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
  • Information by UniProt
  • Database links
  • Alternative names
    • APAF-3 antibody
    • APAF3 antibody
    • Apoptosis related cysteine peptidase antibody
    • Apoptotic protease Mch-6 antibody
    • Apoptotic protease-activating factor 3 antibody
    • CASP-9 antibody
    • CASP9 antibody
    • CASP9_HUMAN antibody
    • Caspase 9 apoptosis related cysteine peptidase antibody
    • Caspase 9 Dominant Negative antibody
    • Caspase 9c antibody
    • Caspase-9 antibody
    • Caspase-9 subunit p10 antibody
    • ICE LAP6 antibody
    • ICE like apoptotic protease 6 antibody
    • ICE-LAP6 antibody
    • ICE-like apoptotic protease 6 antibody
    • MCH6 antibody
    • PPP1R56 antibody
    • protein phosphatase 1, regulatory subunit 56 antibody
    • RNCASP9 antibody
    see all

Anti-Caspase-9 (phospho S196) antibody images

  • All lanes : Anti-Caspase-9 (phospho S196) antibody (ab61789) at 1/500 dilution

    Lane 1 : Extracts from 293 cells treated with Calyculin (50nM, 30mins)
    Lane 2 : Extracts from 293 cells treated with Calyculin (50nM, 30mins) and with the immunising phosphopeptide


    Predicted band size : 46 kDa
    Observed band size : 46 kDa

References for Anti-Caspase-9 (phospho S196) antibody (ab61789)

This product has been referenced in:
  • Dar AA  et al. miRNA-205 suppresses melanoma cell proliferation and induces senescence via regulation of E2F1 protein. J Biol Chem 286:16606-14 (2011). WB ; Human . Read more (PubMed: 21454583) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"