Overview

  • Product nameAnti-Caspr antibody [S65-35]
    See all Caspr primary antibodies
  • Description
    Mouse monoclonal [S65-35] to Caspr
  • Tested applicationsSuitable for: Flow Cyt, WB, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein: QNHRYKGSYH TNEPKATHDS HPGGKAPLPP SGPAQAPAPT PAPTQVPTPA PAPASGPGPR DQNLPQILEE SRSE, corresponding to C terminal (cytoplasmic domain) amino acids 1308-1381 of Rat Caspr (P97846).

  • Positive control
    • Rat brain lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberS65-35
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab105571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 220 kDa (predicted molecular weight: 156 kDa).
IHC-P Use a concentration of 0.1 - 1 µg/ml.
IHC-Fr Use a concentration of 0.1 - 1 µg/ml.
ICC/IF Use a concentration of 0.1 - 10 µg/ml.

Target

  • FunctionSeems to play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the paranodal region of the axo-glial junction. In association with contactin may have a role in the signaling between axons and myelinating glial cells.
  • Tissue specificityPredominantly expressed in brain. Weak expression detected in ovary, pancreas, colon, lung, heart, intestine and testis.
  • Sequence similaritiesBelongs to the neurexin family.
    Contains 2 EGF-like domains.
    Contains 1 F5/8 type C domain.
    Contains 1 fibrinogen C-terminal domain.
    Contains 4 laminin G-like domains.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Caspr antibody
    • Caspr1 antibody
    • CNTNAP antibody
    • Cntnap1 antibody
    • CNTP1_HUMAN antibody
    • Contactin associated protein 1 antibody
    • Contactin-associated protein 1 antibody
    • MHDNIV antibody
    • NCP1 antibody
    • Neurexin 4 antibody
    • Neurexin IV antibody
    • Neurexin-4 antibody
    • Nrxn4 antibody
    • p190 antibody
    • Paranodin antibody
    see all

Anti-Caspr antibody [S65-35] images

  • ab105571 staining Caspr in Mouse brain tissue sections by Immunohistochemistry.

  • ab105571 staining Caspr in Mouse brain tissue sections by Immunohistochemistry.

  • Anti-Caspr antibody [S65-35] (ab105571) at 1/1000 dilution + Rat brain membrane tissue lysate

    Predicted band size : 156 kDa
  • Overlay histogram showing SH-SY5Y cells stained with ab105571 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab105571, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References for Anti-Caspr antibody [S65-35] (ab105571)

This product has been referenced in:
  • Lourbopoulos A  et al. Changes in thyroid hormone receptors after permanent cerebral ischemia in male rats. J Mol Neurosci 54:78-91 (2014). Read more (PubMed: 24577884) »
  • Payne SC  et al. Early proliferation does not prevent the loss of oligodendrocyte progenitor cells during the chronic phase of secondary degeneration in a CNS white matter tract. PLoS One 8:e65710 (2013). IHC ; Rat . Read more (PubMed: 23776532) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"