Overview

  • Product nameAnti-Caspr2 antibody
    See all Caspr2 primary antibodies
  • Description
    Rabbit polyclonal to Caspr2
  • Tested applicationsSuitable for: ICC/IF, IHC-FoFr, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1300 to the C-terminus of Mouse Caspr2.

    (Peptide available as ab34157.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Mouse Brain, Rat Brain and Human Brain

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-FoFr 1/300.
WB Use a concentration of 1 mg/ml. Detects a band of approximately 152 kDa (predicted molecular weight: 148 kDa).
IP Use at an assay dependent concentration.

Target

  • FunctionMay play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the juxtaparanodal region of the axo-glial junction.
  • Tissue specificityPredominantly expressed in nervous system.
  • Involvement in diseaseDefects in CNTNAP2 are the cause of cortical dysplasia-focal epilepsy syndrome (CDFES) [MIM:610042]. Affected individuals manifest cortical dysplasia, focal epilepsy, relative macrocephaly, and diminished deep-tendon reflexes. Intractable focal seizures begin in early childhood, after which language regression, hyperactivity, impulsive and aggressive behavior, and mental retardation develop.
    Genetic variations in CNTNAP2 influences susceptibility to autism type 15 (AUTS15) [MIM:612100]. Autism is a neurodevelopmental disorder characterized by disturbance in language, perception and socialization. The disorder is classically defined by a triad of limited or absent verbal communication, a lack of reciprocal social interaction or responsiveness, and restricted, stereotypical, and ritualized patterns of interests and behavior.
    Note=A chromosomal aberration involving CNTNAP2 is found in a patient with autism spectrum disorder. Paracentric inversion 46,XY,inv(7)(q11.22;q35). The inversion breakpoints disrupt the genes AUTS2 and CNTNAP2.
  • Sequence similaritiesBelongs to the neurexin family.
    Contains 2 EGF-like domains.
    Contains 1 F5/8 type C domain.
    Contains 1 fibrinogen C-terminal domain.
    Contains 4 laminin G-like domains.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AUTS15 antibody
    • CDFE antibody
    • Cell recognition molecule Caspr2 antibody
    • CNTNAP2 antibody
    • CNTP2 antibody
    • CNTP2_HUMAN antibody
    • Contactin-associated protein-like 2 antibody
    • Homolog of Drosophila neurexin IV antibody
    • NRXN4 antibody
    • PTHSL1 antibody
    see all

Anti-Caspr2 antibody images

  • All lanes : Anti-Caspr2 antibody (ab33994) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate at 20 µg
    Lane 2 : Brain (Rat) Tissue Lysate at 20 µg
    Lane 3 : Brain (Human) Tissue Lysate at 20 µg
    Lane 4 : Sciatic Nerve (Mouse) Lysate at 7.5 µg
    Lane 5 : Sciatic Nerve (Rat) Lysate at 20 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 148 kDa
    Observed band size : 152 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 52 kDa. We are unsure as to the identity of these extra bands.
  • Caspr2 was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to Caspr2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab33994.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 152kDa: Caspr2.
  • ICC/IF image of ab33994 stained SHSY5Y cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab33994, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

References for Anti-Caspr2 antibody (ab33994)

This product has been referenced in:

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (brain sections)
Specification brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

Submitted Oct 08 2008

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (brain sections)
Specification brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

Submitted Oct 08 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"