Overview

  • Product nameAnti-Caspr2 antibody
    See all Caspr2 primary antibodies
  • Description
    Rabbit polyclonal to Caspr2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    CDPNFTETIDESKKEWLI

    , corresponding to amino acids 1315-1331 of Human Caspr2, with an additional N terminal cysteine

  • Positive control
    • Rat brain membranes

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.025% Sodium Azide
    Constituents: 5% Sucrose, 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/1000. Predicted molecular weight: 148 kDa.
    Note: Antibody dilutions should be made in buffer containing 1-3% bovine serum albumin.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the juxtaparanodal region of the axo-glial junction.
    • Tissue specificityPredominantly expressed in nervous system.
    • Involvement in diseaseDefects in CNTNAP2 are the cause of cortical dysplasia-focal epilepsy syndrome (CDFES) [MIM:610042]. Affected individuals manifest cortical dysplasia, focal epilepsy, relative macrocephaly, and diminished deep-tendon reflexes. Intractable focal seizures begin in early childhood, after which language regression, hyperactivity, impulsive and aggressive behavior, and mental retardation develop.
      Genetic variations in CNTNAP2 influences susceptibility to autism type 15 (AUTS15) [MIM:612100]. Autism is a neurodevelopmental disorder characterized by disturbance in language, perception and socialization. The disorder is classically defined by a triad of limited or absent verbal communication, a lack of reciprocal social interaction or responsiveness, and restricted, stereotypical, and ritualized patterns of interests and behavior.
      Note=A chromosomal aberration involving CNTNAP2 is found in a patient with autism spectrum disorder. Paracentric inversion 46,XY,inv(7)(q11.22;q35). The inversion breakpoints disrupt the genes AUTS2 and CNTNAP2.
    • Sequence similaritiesBelongs to the neurexin family.
      Contains 2 EGF-like domains.
      Contains 1 F5/8 type C domain.
      Contains 1 fibrinogen C-terminal domain.
      Contains 4 laminin G-like domains.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • AUTS15 antibody
      • CDFE antibody
      • Cell recognition molecule Caspr2 antibody
      • CNTNAP2 antibody
      • CNTP2 antibody
      • CNTP2_HUMAN antibody
      • Contactin-associated protein-like 2 antibody
      • Homolog of Drosophila neurexin IV antibody
      • NRXN4 antibody
      • PTHSL1 antibody
      see all

    Anti-Caspr2 antibody images

    • All lanes : Anti-Caspr2 antibody (ab50206) at 1/1000 dilution

      Lane 1 : Normal rat lung homogenate
      Lane 2 : Normal rat lung plasma membrane fraction
      Lane 3 : Tumor (13762) rat lung homogenate
      Lane 4 : Tumor (13762) rat lung plasma membrane fraction
      Lane 5 : Rat brain homogenate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP conjugated donkey anti-rabbit
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 148 kDa
      Observed band size : 140 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 seconds

      This image is courtesy of an anonymous Abreview

      See Abreview

    References for Anti-Caspr2 antibody (ab50206)

    ab50206 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Tissue lysate - whole (Brain, Tumor Lung (13762) and Normal Lung)
    Loading amount 10 µg
    Specification Brain, Tumor Lung (13762) and Normal Lung
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    Submitted Nov 26 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"