Overview

  • Product name
  • Description
    Rabbit polyclonal to Caspr2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    CDPNFTETIDESKKEWLI

    , corresponding to amino acids 1315-1331 of Human Caspr2, with an additional N terminal cysteine

  • Positive control
    • Rat brain membranes

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.025% Sodium Azide
    Constituents: 5% Sucrose, 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/1000. Predicted molecular weight: 148 kDa.
    Note: Antibody dilutions should be made in buffer containing 1-3% bovine serum albumin.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      May play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the juxtaparanodal region of the axo-glial junction.
    • Tissue specificity
      Predominantly expressed in nervous system.
    • Involvement in disease
      Defects in CNTNAP2 are the cause of cortical dysplasia-focal epilepsy syndrome (CDFES) [MIM:610042]. Affected individuals manifest cortical dysplasia, focal epilepsy, relative macrocephaly, and diminished deep-tendon reflexes. Intractable focal seizures begin in early childhood, after which language regression, hyperactivity, impulsive and aggressive behavior, and mental retardation develop.
      Genetic variations in CNTNAP2 influences susceptibility to autism type 15 (AUTS15) [MIM:612100]. Autism is a neurodevelopmental disorder characterized by disturbance in language, perception and socialization. The disorder is classically defined by a triad of limited or absent verbal communication, a lack of reciprocal social interaction or responsiveness, and restricted, stereotypical, and ritualized patterns of interests and behavior.
      Note=A chromosomal aberration involving CNTNAP2 is found in a patient with autism spectrum disorder. Paracentric inversion 46,XY,inv(7)(q11.22;q35). The inversion breakpoints disrupt the genes AUTS2 and CNTNAP2.
    • Sequence similarities
      Belongs to the neurexin family.
      Contains 2 EGF-like domains.
      Contains 1 F5/8 type C domain.
      Contains 1 fibrinogen C-terminal domain.
      Contains 4 laminin G-like domains.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • AUTS15 antibody
      • CDFE antibody
      • Cell recognition molecule Caspr2 antibody
      • CNTNAP2 antibody
      • CNTP2 antibody
      • CNTP2_HUMAN antibody
      • Contactin-associated protein-like 2 antibody
      • Homolog of Drosophila neurexin IV antibody
      • NRXN4 antibody
      • PTHSL1 antibody
      see all

    Anti-Caspr2 antibody images

    • All lanes : Anti-Caspr2 antibody (ab50206) at 1/1000 dilution

      Lane 1 : Normal rat lung homogenate
      Lane 2 : Normal rat lung plasma membrane fraction
      Lane 3 : Tumor (13762) rat lung homogenate
      Lane 4 : Tumor (13762) rat lung plasma membrane fraction
      Lane 5 : Rat brain homogenate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP conjugated donkey anti-rabbit
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 148 kDa
      Observed band size : 140 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 seconds

      This image is courtesy of an anonymous Abreview

      See Abreview

    References for Anti-Caspr2 antibody (ab50206)

    ab50206 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Brain, Tumor Lung (13762) and Normal Lung)
    Loading amount
    10 µg
    Specification
    Brain, Tumor Lung (13762) and Normal Lung
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    Submitted Nov 26 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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