Recombinant
RabMAb

Anti-CASS4 antibody [EPR8947(2)] (ab157198)

Overview

  • Product name
    Anti-CASS4 antibody [EPR8947(2)]
    See all CASS4 primary antibodies
  • Description
    Rabbit monoclonal [EPR8947(2)] to CASS4
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human CASS4 (UniProt: Q9NQ75).

  • Positive control
    • Human fetal lung, K562, and MDA-MB-435 cell lysates; Human ovarian carcinoma and Human spleen tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated "PUR" on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab157198 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 87 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Possible docking protein which may play a role for tyrosine-kinase-based signaling related to cell adhesion. Regulates FAK activity, focal adhesion integrity, and cell spreading.
    • Tissue specificity
      Expressed abundantly in lung and spleen. Also highly expressed in ovarian and leukemia cell lines.
    • Sequence similarities
      Belongs to the CAS family.
      Contains 1 SH3 domain.
    • Post-translational
      modifications
      Phosphorylated on tyrosines by SRC.
    • Cellular localization
      Cytoplasm > cytoskeleton. Cell junction > focal adhesion.
    • Information by UniProt
    • Database links
    • Alternative names
      • C20orf32 antibody
      • Cas scaffolding protein family member 4 antibody
      • CAS4 antibody
      • CASS4 antibody
      • CASS4_HUMAN antibody
      • HEF-like protein antibody
      • HEF1-EFS-p130Cas-like protein antibody
      • HEFL antibody
      • HEPL antibody
      • OTTHUMP00000031328 antibody
      • OTTHUMP00000031329 antibody
      see all

    Images

    • All lanes : Anti-CASS4 antibody [EPR8947(2)] (ab157198) at 1/1000 dilution

      Lane 1 : Human fetal lung lysate
      Lane 2 : K562 lysate
      Lane 3 : MDA-MB-435 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 87 kDa
    • Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling CASS4 with ab157198 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CASS4 with ab157198 at 1/50 dilution.

    References

    ab157198 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab157198.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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