Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464)


  • Product name
    Catalase Activity Assay Kit (Colorimetric/Fluorometric)
    See all Catalase kits
  • Sample type
    Urine, Serum, Plasma, Cell Lysate, Tissue Lysate
  • Assay type
  • Assay time
    0h 40m
  • Product overview

    Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464) is a highly sensitive, simple and direct assay for measuring catalase activity in a variety of biological samples such as cell and tissue lysates or biological fluids. In this assay, the catalase present in the sample reacts with hydrogen peroxide (H2O2) to produce water and oxygen. The unconverted H2O2 reacts with probe to produce a product that can be measured colorimetrically at OD 570 nm or fluorometrically at Ex/Em = 535/587 nm. Therefore, the catalase activity present in the sample is reversely proportional to the signal obtained. The kit can detect as little as 1 µU of catalase activity.

    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Catalase (EC is a ubiquitous antioxidant enzyme that is present in nearly all living organisms. It catalyzes the decomposition of hydrogen peroxide (H2O2) to water and oxygen. Catalase is a tetramer of four polypeptide chains, and contains four porphyrin heme (iron) groups that allow the enzyme to react with hydrogen peroxide. The optimum pH for human catalase is approximately pH 7, with a fairly broad maximum as the rate of reaction does not change appreciably between pH= 6.8-7.5.

  • Tested applications
    Suitable for: Functional Studiesmore details


  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components Identifier 100 tests
    Catalase Assay Buffer NM 1 x 25ml
    Catalase Positive Control Blue 1 x 2µl
    HRP (Lyophilised) Green 1 vial
    Hydrogen peroxide Yellow 1 x 25µl
    OxiRed Probe Red 1 x 200µl
    Stop Solution White 1 x 1ml
  • Research areas
  • Function
    Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
  • Involvement in disease
    Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
  • Sequence similarities
    Belongs to the catalase family.
  • Post-translational
    The N-terminus is blocked.
  • Cellular localization
  • Information by UniProt
  • Alternative names
    • CAT
    • Catalase
    • EC
    • MGC138422
    • MGC138424
    see all

Associated products


Our Abpromise guarantee covers the use of ab83464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.


  • H2O2 standard curve using ab83464.
  • Sample tests using ab83464.



This product has been referenced in:
  • Cases J  et al. Supplementation with a Polyphenol-Rich Extract, PerfLoad(®), Improves Physical Performance during High-Intensity Exercise: A Randomized, Double Blind, Crossover Trial. Nutrients 9:N/A (2017). Functional Studies ; Human . Read more (PubMed: 28441760) »
  • Kim HY  et al. Effect of fermented porcine placenta on physical fatigue in mice. Exp Biol Med (Maywood) 241:1985-1996 (2016). Functional Studies ; Mouse . Read more (PubMed: 27439540) »

See all 8 Publications for this product

Customer reviews and Q&As

Regarding the HRP  The amount of HRP once reconstituted in 200 ul is enough for 1 plate + 1 extra reaction (2 ul per reaction). This should therefore be enough for a 1 plate assay.   The 1 extra reaction amount means that if for example you want to spl...

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We have added the stop solution for ab83464 to our catalog as ab176555. A link to the product datasheet is given below:

The High Control (HC) is a well that has the same volume of sample (2-78 uL) loaded as your sample wells. If you load wells with different volumes of sample, you will need to also load multiple HC wells with the same volume of sample. Prior to add...

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Gracias por contactarnos.

En el kit tiene lugar la reacción de H2O2 para producir H2O y oxigeno catalizado por la enzima catalasa. El H2O2 que queda sin reaccionar reacciona con OxiRed™ generando un producto que presenta absorban...

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Thank you for contacting us.

I can confirm that the fluorimetric detection method can be used with the 545/590 filter. Please note that the expected results could be of less quality as reading results with a 535/587 filter would be.


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Thank you for contacting us. I am sorry to hear that you have had problems with our Catalase Assay Kit (ab83464)

I would like to reassure you that this kit is tested and covered by our 6 month guarantee for Tissue extracts. In the event that ...

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Thank you very much for your interest in our products.

To our knowledge, we have do not have a Catalase Assay kit in our catalog that is tested and guaranteed for plants. Since ab83464 is working by measuring the the unconverted H2O2, w...

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Thank you for your reply.

As for your samples, the dilution is extremely important and from your previous results it seems that 1:20 is best of the three you have already tested. Perhaps testing 1:25 and/or 1:30 would also be useful to ch...

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Thank you for your patience as I have investigated this issue further with the laboratory.

I can confirm that you performed the assay correctly the second time, only adding stop solution to the standards and HC ONCE.

It is n...

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The HC and standard wells already contain the stop solution. You need NOT add any more stop solution to these wells. Add the stop solution only to the sample wells in step 3 of this protocol.

Hope this information has been helpful for y...

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1-10 of 30 Abreviews or Q&A


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