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Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464) is a highly sensitive, simple and direct assay for measuring catalase activity in a variety of biological samples such as cell and tissue lysates or biological fluids. In this assay, the catalase present in the sample reacts with hydrogen peroxide (H2O2) to produce water and oxygen. The unconverted H2O2 reacts with probe to produce a product that can be measured colorimetrically at OD 570 nm or fluorometrically at Ex/Em = 55/587 nm. Therefore, the catalase activity present in the sample is reversely proportional to the signal obtained. The kit can detect as little as 1 µU of catalase activity.
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Catalase (EC 184.108.40.206) is a ubiquitous antioxidant enzyme that is present in nearly all living organisms. It catalyzes the decomposition of hydrogen peroxide (H2O2) to water and oxygen. Catalase is a tetramer of four polypeptide chains, and contains four porphyrin heme (iron) groups that allow the enzyme to react with hydrogen peroxide. The optimum pH for human catalase is approximately pH 7, with a fairly broad maximum as the rate of reaction does not change appreciably between pH= 6.8-7.5.
|Catalase Assay Buffer||NM||1 x 25ml|
|Catalase Positive Control||Blue||1 x 2µl|
|HRP (Lyophilised)||Green||1 vial|
|Hydrogen peroxide||Yellow||1 x 25µl|
|OxiRed Probe||Red||1 x 200µl|
|Stop Solution||White||1 x 1ml|
Our Abpromise guarantee covers the use of ab83464 in the following tested applications.
|Functional Studies||Use at an assay dependent dilution.|