Anti-Catalase antibody [12C2DB9 ] - Peroxisome Marker (ab110292)

  Immunocytochemistry - Anti-Catalase antibody [12C2DB9 ] (ab110292)

Immunocytochemical staining of Human HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the ab110292 (5 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody, Alexa Fluor® 488 goat anti-mouse IgG (green), was used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). The target protein locates mainly in peroxisomes.

  Immunoprecipitation - Anti-Catalase antibody [12C2DB9 ] (ab110292)

Immunoprecipitation of Catalase using ab110292 from:
Lane 1: HepG2 cells
Lane 2: Cow heart sample
Lane 3: Rat liver sample

Predicted band size is 60 kDa.

  Flow Cytometry - Catalase antibody [12C2DB9 ] (ab110292)

Flow cytometric analysis of HL-60 cells using ab110292 at 1 µg/mL, (blue) or an isotype control (red).