MitoSciences (MS721)

Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)

Overview

  • Product nameAnti-Catalase antibody [12C2DB9] - Peroxisome MarkerSee all Catalase primary antibodies ...
  • Description
    Mouse monoclonal [12C2DB9] to Catalase - Peroxisome Marker
  • Tested applicationsICC, IP, Flow Cyt, In-Cell ELISAmore details
  • Species reactivity
    Reacts with: Rat, Cow, Human
  • Immunogen

    Rat liver mitochondria

  • Positive control
    • HeLa cells, HepG2 cells, HL60 cells, Cow heart, Rat liver

Properties

Applications

Our Abpromise guarantee covers the use of ab110292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 5 µg/ml.
IP Use at an assay dependent dilution.
Flow Cyt Use a concentration of 1 µg/ml.
In-Cell ELISA Use a concentration of 4 µg/ml.

Target

  • FunctionOccurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
  • Involvement in diseaseDefects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
  • Sequence similaritiesBelongs to the catalase family.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localizationPeroxisome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cas1 antibody
    • CAT antibody
    • CATA_HUMAN antibody
    • Catalase antibody
    • Cs1 antibody
    • MGC138422 antibody
    • MGC138424 antibody
    see all

Anti-Catalase antibody [12C2DB9] - Peroxisome Marker images

  • Immunocytochemical staining of Human HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the ab110292 (5 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody, Alexa Fluor® 488 goat anti-mouse IgG (green), was used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). The target protein locates mainly in peroxisomes.
  • Immunoprecipitation of Catalase using ab110292 from:
    Lane 1: HepG2 cells
    Lane 2: Cow heart sample
    Lane 3: Rat liver sample

    Predicted band size is 60 kDa.
  • Flow cytometric analysis of HL-60 cells using ab110292 at 1 µg/mL, (blue) or an isotype control (red).

References for Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)

ab110292 has not yet been referenced specifically in any publications.

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Unfortunately, the dissociation constant KD is not routinely determined and we therefore cannot provide this information on our antibodies against bovine catalase.

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