Anti-Catalase antibody [1B6] (ab125688)

Overview

  • Product nameAnti-Catalase antibody [1B6]
    See all Catalase primary antibodies
  • Description
    Mouse monoclonal [1B6] to Catalase
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Dog, Human, Monkey
  • Immunogen

    Recombinant full length Human Catalase produced in HEK293T cells (NP_111743).

  • Positive control
    • Catalase-transfected HEK293T cell lysate; HepG2, HeLa, HT29, A549, COS7, Jurkat, MDCK, PC12, and MCF7 cell extracts; Human Kidney, liver, lung, endometrium, prostate, prostate carcinoma, lymph node and lymphoma tissues; COS7 cells transiently transfected by Catalase.
  • General notesDilute in PBS (pH7.3) before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab125688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/500. Predicted molecular weight: 60 kDa.
IHC-P 1/150.
ICC/IF 1/100.
Flow Cyt 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionOccurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
  • Involvement in diseaseDefects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
  • Sequence similaritiesBelongs to the catalase family.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localizationPeroxisome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cas1 antibody
    • CAT antibody
    • CATA_HUMAN antibody
    • Catalase antibody
    • Cs1 antibody
    • MGC138422 antibody
    • MGC138424 antibody
    see all

Anti-Catalase antibody [1B6] images



  • Predicted band size : 60 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Empty 
    Lane 3: Catalase knockout HAP1 cell lysate (20 µg)
    Lane 4: Empty
    Lanes 1 - 4: Merged signal (red and green). Green - ab125688 observed at 60 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab125688 was shown to specifically react with Catalase when Catalase knockout samples were used. Wild-type and Catalase knockout samples were subjected to SDS-PAGE. ab125688 and ab181602 (loading control to GAPDH) were diluted 1/200 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Overlay histogram showing HeLa cells stained with ab125688 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab125688, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • ab125688, at a dilution of 1/150, staining Catalase in paraffin-embedded Human kidney carcinoma tissue by Immunohistochemistry.
  • ab125688, at a dilution of 1/150, staining Catalase in paraffin-embedded Human liver tissue by Immunohistochemistry.
  • All lanes : Anti-Catalase antibody [1B6] (ab125688) at 1/200 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Catalase cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 60 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC210763)
  • ab125688, at a dilution of 1/150, staining Catalase in paraffin-embedded Human lung tissue by Immunohistochemistry.
  • ab125688, at a dilution of 1/150, staining Catalase in paraffin-embedded Human endometrium tissue by Immunohistochemistry.
  • ab125688, at a dilution of 1/150, staining Catalase in paraffin-embedded Human prostate tissue by Immunohistochemistry.
  • All lanes : Anti-Catalase antibody [1B6] (ab125688) at 1/200 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : HT29 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 60 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC210763)
  • ab125688, at a dilution of 1/150, staining Catalase in paraffin-embedded Human prostate carcinoma tissue by Immunohistochemistry.
  • ab125688, at a dilution of 1/150, staining Catalase in paraffin-embedded Human lymph node by Immunohistochemistry.
  • ab125688, at a dilution of 1/150, staining Catalase in paraffin-embedded Human lymphoma tissue by Immunohistochemistry.
  • ab125688, at 1/100 dilution, staining Catalase in COS7 cells transiently transfected by pCMV6-ENTRY Catalase by Immunofluorescence.

References for Anti-Catalase antibody [1B6] (ab125688)

ab125688 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Sample Rabbit Tissue sections (Lung)
Specification Lung
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 29 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"