Overview

  • Product name
  • Description
    Goat polyclonal to Catalase
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human, Pig
    Predicted to work with: Mouse, Guinea pig, Cow, Dog, Common marmoset, Orangutan
  • Immunogen

    Synthetic peptide:

    C-DKYNAEKPKNAIHT

    , corresponding to internal sequence amino acids 498-511 of Human Catalase

  • Positive control
    • Human Kidney and Liver lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab50434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
IP Use at an assay dependent dilution. PubMed: 20923778
IHC-P Use a concentration of 2 - 4 µg/ml.

Target

  • Function
    Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
  • Involvement in disease
    Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
  • Sequence similarities
    Belongs to the catalase family.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localization
    Peroxisome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cas1 antibody
    • CAT antibody
    • CATA_HUMAN antibody
    • Catalase antibody
    • Cs1 antibody
    • MGC138422 antibody
    • MGC138424 antibody
    see all

Anti-Catalase antibody images

  • Anti-Catalase antibody (ab50434) at 1 µg/ml + Human Kidney lysate (35µg protein in RIPA buffer).

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
  • ab50434 at 2µg/ml staining Human liver tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue underwent antigen retrieval by steam in citrate buffer pH 6.0. The AP staining procedure was used.

References for Anti-Catalase antibody (ab50434)

This product has been referenced in:
  • Xie W  et al. Exercise training-enhanced, endothelium-dependent dilation mediated by altered regulation of BK(Ca) channels in collateral-dependent porcine coronary arterioles. Microcirculation 20:170-82 (2013). Read more (PubMed: 23002811) »
  • Habib LK  et al. Inhibitors of Catalase-Amyloid Interactions Protect Cells from {beta}-Amyloid-Induced Oxidative Stress and Toxicity. J Biol Chem 285:38933-43 (2010). IP ; Human . Read more (PubMed: 20923778) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Pig Tissue lysate - whole (coronary arteries and arterioles)
Loading amount
20 µg
Specification
coronary arteries and arterioles
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2.5µg/mL · Temperature: 37°C
Username

Dr. Wei Xie

Verified customer

Submitted Jul 13 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up