Overview

  • Product name
  • Description
    Rabbit polyclonal to Catalase
  • Specificity
    This antibody reacts with Catalase
  • Tested applications
    Suitable for: WB, IP, ICC, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N terminus of human Catalase

  • Positive control
    • IHC-P: Human liver tissue WB: TF1 lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab52477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
IP 1/10.
ICC 1/50 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
    • Involvement in disease
      Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
    • Sequence similarities
      Belongs to the catalase family.
    • Post-translational
      modifications
      The N-terminus is blocked.
    • Cellular localization
      Peroxisome.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cas1 antibody
      • CAT antibody
      • CATA_HUMAN antibody
      • Catalase antibody
      • Cs1 antibody
      • MGC138422 antibody
      • MGC138424 antibody
      see all

    Anti-Catalase antibody images



    • Predicted band size : 60 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Catalase knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab52477 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab52477 was shown to specifically react with Catalase when Catalase knockout samples were used. Wild-type and Catalase knockout samples were subjected to SDS-PAGE. ab52477 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) andGoat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Anti-Catalase antibody (ab52477) at 1/1000 dilution + TF1 lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 60 kDa
      Observed band size : 60 kDa
    • IHC image of Catalasestaining in a formalin fixed, paraffin embedded normal human liver tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52477, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

       

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • All lanes : Anti-Catalase antibody (ab52477) at 1 µg/ml

      Lane 1 : Whole cell lysate prepared from 293T cells
      Lane 2 : Whole cell lysate prepared from HeLa cells
      Lane 3 : Whole cell lysate prepared from U251 cells
      Lane 4 : Whole cell lysate prepared from Jurkat cells
      Lane 5 : Whole cell lysate prepared from U2OS cells

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP conjugated pig anti-rabbit polyclonal at 1/5000 dilution
      Developed using the ECL technique

      Predicted band size : 60 kDa
      Observed band size : 60 kDa


      Exposure time : 5 minutes

      Image courtesy of an anonymous Abreview.

      See Abreview

    • All lanes : Anti-Catalase antibody (ab52477) at 1 µg/ml

      Lane 1 : Whole cell lysate prepared from 9L cells
      Lane 2 : Whole cell lysate prepared from PC12 cells
      Lane 3 : Whole cell lysate prepared from RIE-1 cells

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP conjugated pig anti-rabbit polyclonal at 1/5000 dilution
      Developed using the ECL technique

      Predicted band size : 60 kDa
      Observed band size : 60 kDa


      Exposure time : 5 minutes

      Image courtesy of an anonymous Abreview.

      See Abreview

    References for Anti-Catalase antibody (ab52477)

    This product has been referenced in:
    • Ahn JH  et al. Comparison of catalase immunoreactivity in the hippocampus between young, adult and aged mice and rats. Mol Med Rep 14:851-6 (2016). IHC ; Mouse, Rat . Read more (PubMed: 27221506) »
    • Peng S  et al. In Barrett's esophagus patients and Barrett's cell lines, ursodeoxycholic acid increases antioxidant expression and prevents DNA damage by bile acids. Am J Physiol Gastrointest Liver Physiol 307:G129-39 (2014). WB ; Human . Read more (PubMed: 24852569) »

    See all 9 Publications for this product

    Product Wall

    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (hepatocytes)
    Specification
    hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 14 2015

    Application
    Western blot
    Loading amount
    20000 cells
    Gel Running Conditions
    Reduced Denaturing (10%)
    Sample
    Human Cell lysate - whole cell (jurkats)
    Specification
    jurkats
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Dr. Tejpal Gill

    Verified customer

    Submitted Jan 13 2014

    Thank you for your reply.

    We have a couple of other catalase antibodies that seem to work well, ab1877 or ab16731-

    http://www.abcam.com/Catalase-antibody-Peroxisome-Marker-ab1877.html

    http://www.abcam.com/Catalase-antibody...

    Read More

    Thank you for contacting us and letting us know about the trouble with this antibody. I am sorry to hear that the results have not been satisfactory.

    I looked through our records and found the previous correspondence you had with my colleague...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Rat cell lines)
    Loading amount
    20 µg
    Specification
    Rat cell lines
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 20 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human cancer cell lines)
    Loading amount
    20 µg
    Specification
    Human cancer cell lines
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 20 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (MEF F9 GC1 NIH-3T3)
    Loading amount
    20 µg
    Specification
    MEF F9 GC1 NIH-3T3
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 02 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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