Overview

  • Product nameAnti-Catalase antibody
    See all Catalase primary antibodies
  • Description
    Rabbit polyclonal to Catalase
  • SpecificityThis antibody reacts with Catalase
  • Tested applicationsSuitable for: WB, IP, ICC, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N terminus of human Catalase

  • Positive control
    • IHC-P: Human liver tissue WB: TF1 lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab52477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
IP 1/10.
ICC 1/50 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionOccurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
    • Involvement in diseaseDefects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
    • Sequence similaritiesBelongs to the catalase family.
    • Post-translational
      modifications
      The N-terminus is blocked.
    • Cellular localizationPeroxisome.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cas1 antibody
      • CAT antibody
      • CATA_HUMAN antibody
      • Catalase antibody
      • Cs1 antibody
      • MGC138422 antibody
      • MGC138424 antibody
      see all

    Anti-Catalase antibody images



    • Predicted band size : 60 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Catalase knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab52477 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab52477 was shown to specifically react with Catalase when Catalase knockout samples were used. Wild-type and Catalase knockout samples were subjected to SDS-PAGE. ab52477 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Anti-Catalase antibody (ab52477) at 1/1000 dilution + TF1 lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 60 kDa
      Observed band size : 60 kDa
    • Immunohistochemical staining of paraffin-embedded human liver using ab52477 at a 1/100 dilution.
    • All lanes : Anti-Catalase antibody (ab52477) at 1 µg/ml

      Lane 1 : Whole cell lysate prepared from 293T cells
      Lane 2 : Whole cell lysate prepared from HeLa cells
      Lane 3 : Whole cell lysate prepared from U251 cells
      Lane 4 : Whole cell lysate prepared from Jurkat cells
      Lane 5 : Whole cell lysate prepared from U2OS cells

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP conjugated pig anti-rabbit polyclonal at 1/5000 dilution
      Developed using the ECL technique

      Predicted band size : 60 kDa
      Observed band size : 60 kDa


      Exposure time : 5 minutes

      Image courtesy of an anonymous Abreview.

      See Abreview

    • All lanes : Anti-Catalase antibody (ab52477) at 1 µg/ml

      Lane 1 : Whole cell lysate prepared from 9L cells
      Lane 2 : Whole cell lysate prepared from PC12 cells
      Lane 3 : Whole cell lysate prepared from RIE-1 cells

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP conjugated pig anti-rabbit polyclonal at 1/5000 dilution
      Developed using the ECL technique

      Predicted band size : 60 kDa
      Observed band size : 60 kDa


      Exposure time : 5 minutes

      Image courtesy of an anonymous Abreview.

      See Abreview

    References for Anti-Catalase antibody (ab52477)

    This product has been referenced in:
    • Peng S  et al. In Barrett's esophagus patients and Barrett's cell lines, ursodeoxycholic acid increases antioxidant expression and prevents DNA damage by bile acids. Am J Physiol Gastrointest Liver Physiol 307:G129-39 (2014). WB ; Human . Read more (PubMed: 24852569) »
    • Huang WC  et al. Activation of androgen receptor, lipogenesis, and oxidative stress converged by SREBP-1 is responsible for regulating growth and progression of prostate cancer cells. Mol Cancer Res 10:133-42 (2012). WB ; Human . Read more (PubMed: 22064655) »

    See all 8 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 25 µg
    Gel Running Conditions Reduced Denaturing
    Sample Human Cell lysate - whole cell (hepatocytes)
    Specification hepatocytes
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 14 2015

    Application Western blot
    Loading amount 20000 cells
    Gel Running Conditions Reduced Denaturing (10%)
    Sample Human Cell lysate - whole cell (jurkats)
    Specification jurkats
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Dr. Tejpal Gill

    Verified customer

    Submitted Jan 13 2014

    Thank you for your reply.

    We have a couple of other catalase antibodies that seem to work well, ab1877 or ab16731-

    http://www.abcam.com/Catalase-antibody-Peroxisome-Marker-ab1877.html

    http://www.abcam.com/Catalase-antibody...

    Read More

    Thank you for contacting us and letting us know about the trouble with this antibody. I am sorry to hear that the results have not been satisfactory.

    I looked through our records and found the previous correspondence you had with my colleague...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Cell lysate - whole cell (Rat cell lines)
    Loading amount 20 µg
    Specification Rat cell lines
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 20 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Human cancer cell lines)
    Loading amount 20 µg
    Specification Human cancer cell lines
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 20 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (MEF F9 GC1 NIH-3T3)
    Loading amount 20 µg
    Specification MEF F9 GC1 NIH-3T3
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 02 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"