Overview

  • Product nameAnti-Catalase antibody
    See all Catalase primary antibodies
  • Description
    Mouse polyclonal to Catalase
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human Catalase protein (NP_001743.1).

  • Positive control
    • Human liver tissue lysate. A-431 cell lysate. Cell lysate from transfected 293T cells. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab88650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionOccurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
  • Involvement in diseaseDefects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
  • Sequence similaritiesBelongs to the catalase family.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localizationPeroxisome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cas1 antibody
    • CAT antibody
    • CATA_HUMAN antibody
    • Catalase antibody
    • Cs1 antibody
    • MGC138422 antibody
    • MGC138424 antibody
    see all

Anti-Catalase antibody images

  • Anti-Catalase antibody (ab88650) at 1 µg/ml + human liver tissue lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 60 kDa
  • Anti-Catalase antibody (ab88650) at 1 µg/ml + A431 cell lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 60 kDa
  • All lanes : Anti-Catalase antibody (ab88650) at 1 µg/ml

    Lane 1 : cell lysate from Catalase transfected 293T cells
    Lane 2 : cell lysate from non transfected 293T cells

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
  • ab88650 at 10 µg/ml detecting Catalase in paraformaldehyde fixed, permeabilised HeLa cells by immunofluorescence.

References for Anti-Catalase antibody (ab88650)

This product has been referenced in:
  • Chao MW  et al. N-acetylcysteine attenuates lipopolysaccharide-induced impairment in lamination of Ctip2-and Tbr1- expressing cortical neurons in the developing rat fetal brain. Sci Rep 6:32373 (2016). WB . Read more (PubMed: 27577752) »
  • Pircher H  et al. Identification of Human Fumarylacetoacetate Hydrolase Domain-containing Protein 1 (FAHD1) as a Novel Mitochondrial Acylpyruvase. J Biol Chem 286:36500-8 (2011). ICC/IF ; Human . Read more (PubMed: 21878618) »

See all 2 Publications for this product

Product Wall

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab1877.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"