Overview

  • Product nameAnti-Catalase antibody
    See all Catalase primary antibodies
  • Description
    Rabbit polyclonal to Catalase
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 467-516 (LKDAQIFIQK KAVKNFTEVH PDYGSHIQAL LDKYNAEKPK NAIHTFVQSG) of Human Catalase (NP_001743).

  • Positive control
    • Human fetal heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab98210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration.

Target

Anti-Catalase antibody images

  • Anti-Catalase antibody (ab98210) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 60 kDa
    Gel concentration: 12%
  • ab98210 staining Catalase in murine J774 macrophages by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in 5% paraformaldehyde and permeabilized using ice cold acetone, blocked with 30% human serum, 0.1% BSA, 1% fish gelatin, 5 mM EDTA, and 0.2% Tween 20 in PBS for 30 minutes at 4°C. Samples were then incubated with ab98210 at a 1/100 dilution for 1 hour at 4°C. The secondary used was an Alexa Fluor 488 conjugated antibody used at a 1/1000 dilution. Counterstained with DAPI.

References for Anti-Catalase antibody (ab98210)

ab98210 has not yet been referenced specifically in any publications.

Product Wall

The ab16731 is not specific to N-terminal end of the protein. It is produced using recombinant fragment of whole protein and because it is a polyclonal antibody so it will bind to multiple epitopes. It is likely that ab16731 will be able to detect the ...

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Thank you for sending the image which is very self explanatory for multiple bands. Please ignore my suggestions for multiple bands as these were more general rather than related to this case. The antibody (ab98210) I recommended was the second most...

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Thank you for your email. I am sorry to hear that the results did not improve. The multiple band problem could be due to following reasons: - High protein conc. of lysates per well - High conc. of primary and secondary ab - Cell confluence or ...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this ha...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"