Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)

Overview

  • Product nameAnti-Catalase antibody [EP1929Y] - Peroxisome Marker
    See all Catalase primary antibodies
  • Description
    Rabbit monoclonal [EP1929Y] to Catalase - Peroxisome Marker
  • Tested applicationsSuitable for: WB, ICC, Flow Cyt, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the C terminus of human Catalase

  • Positive control
    • WB: HeLa cell lysate. IHC-P: human brain tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Alternative versions available:

    Anti-Catalase antibody (Alexa Fluor® 488) [EP1929Y] - Peroxisome Marker (ab185041)

Properties

Applications

Our Abpromise guarantee covers the use of ab76024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/20000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ICC 1/100 - 1/250.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionOccurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
    • Involvement in diseaseDefects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
    • Sequence similaritiesBelongs to the catalase family.
    • Post-translational
      modifications
      The N-terminus is blocked.
    • Cellular localizationPeroxisome.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cas1 antibody
      • CAT antibody
      • CATA_HUMAN antibody
      • Catalase antibody
      • Cs1 antibody
      • MGC138422 antibody
      • MGC138424 antibody
      see all

    Anti-Catalase antibody [EP1929Y] - Peroxisome Marker images

    • Overlay histogram showing HeLa cells stained with ab76024 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76024, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024) at 1/20000 dilution + HeLa cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 60 kDa
      Observed band size : 60 kDa
    • Immunohistochemical staining of Catalase in paraffin embedded human normal brain tissue using ab76024 at a 1/100 dilution.

    References for Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)

    This product has been referenced in:
    • Ma HP Hydrogen peroxide stimulates the epithelial sodium channel through a phosphatidylinositide 3-kinase-dependent pathway. J Biol Chem 286:32444-53 (2011). WB ; Xenopus laevis . Read more (PubMed: 21795700) »
    • Kemp K  et al. Mesenchymal stem cells restore frataxin expression and increase hydrogen peroxide scavenging enzymes in friedreich ataxia fibroblasts. PLoS One 6:e26098 (2011). WB ; Human . Read more (PubMed: 22016819) »

    See all 2 Publications for this product

    Product Wall

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this ha...

    Read More
    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (rat intestinal epithelial cells (RIE-1))
    Permeabilization Yes - 0.2% TritonX-100
    Specification rat intestinal epithelial cells (RIE-1)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Feb 12 2016

    Application Flow Cytometry
    Sample Mouse Cell (Mouse Lewis Lung Carcinoma cells)
    Permeabilization Yes - 0.2% Triton X-100
    Gating Strategy No gating
    Specification Mouse Lewis Lung Carcinoma cells
    Preparation Cell harvesting/tissue preparation method: trypsin
    Sample buffer: Hanks’ Balanced Salt solution (HBSS)
    Fixation Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jan 25 2016

    Application IHC - Wholemount
    Sample Human Tissue (Ovary adenocarcinoma)
    Specification Ovary adenocarcinoma
    Username

    Abcam user community

    Verified customer

    Submitted Dec 21 2015

    Application IHC - Wholemount
    Sample Mouse Tissue (Transplanted lung tumor section)
    Specification Transplanted lung tumor section
    Username

    Abcam user community

    Verified customer

    Submitted Dec 21 2015

    Application Western blot
    Sample African Green Monkey Cell lysate - whole cell (COS-7 cells)
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
    Loading amount 100000 cells
    Treatment 30 nM Bortezomib for 24 hours
    Specification COS-7 cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 24 2015

    Application Flow Cytometry
    Sample Human Cell (A549 lung cancer cells)
    Permeabilization Yes - 0.2% Triton X-100
    Gating Strategy No gating
    Specification A549 lung cancer cells
    Preparation Cell harvesting/tissue preparation method: trypsin
    Sample buffer: Hanks’ Balanced Salt solution (HBSS)
    Fixation Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted May 15 2015

    Application Western blot
    Sample Mouse Cell lysate - whole cell (Lewis lung cancer, FL5.12, embryonic fibroblasts)
    Gel Running Conditions Reduced Denaturing (4-20% Bis-Tris)
    Loading amount 100000 cells
    Specification Lewis lung cancer, FL5.12, embryonic fibroblasts
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted May 11 2015

    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (mouse embryonic fibroblasts)
    Permeabilization Yes - 0.2% Triton X-100
    Specification mouse embryonic fibroblasts
    Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 1.5% · Temperature: 25°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted May 05 2015

    Application Western blot
    Sample Human Cell lysate - whole cell (A549 lung cancer cells)
    Gel Running Conditions Reduced Denaturing (4-20% Tris-glycine)
    Loading amount 15 µg
    Specification A549 lung cancer cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 21 2015

    1-10 of 15 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"