The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Application notesIs unsuitable for Flow Cyt,ICC or IP.
FunctionOccurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
Involvement in diseaseDefects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
Sequence similaritiesBelongs to the catalase family.
Post-translational modificationsThe N-terminus is blocked.
Western blot - Anti-Catalase antibody [EPR1928Y] (ab76110)
Predicted band size : 60 kDa
Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: Catalase knockout HAP1 cell lysate (20 µg) Lane 3: HeLa cell lysate (20 µg) Lane 4: Jurkat cell lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab76110 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa. ab76110 was shown to specifically react with Catalase when Catalase knockout samples were used. Wild-type and Catalase knockout samples were subjected to SDS-PAGE. ab76110 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
ab76110 at 1/100-1/250 dilution staining Catalase in human liver by Immunohistochemistry, Paraffin-embedded tissue.
Western blot - Catalase antibody [EPR1928Y] (ab76110)
All lanes : Anti-Catalase antibody [EPR1928Y] (ab76110) at 1/1000 dilution
Lane 1 : TF cell lysate Lane 2 : HeLa cell lysate Lane 3 : Jurkat cell lysate Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 60 kDa Observed band size : 60 kDa
References for Anti-Catalase antibody [EPR1928Y] (ab76110)
This product has been referenced in:
Baqader NO et al. Nuclear cytoplasmic trafficking of proteins is a major response of human fibroblasts to oxidative stress. J Proteome Res13:4398-423 (2014).
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