• Product nameCatalase Assay Kit
    See all Catalase kits
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Assay time
    0h 40m
  • Product overview

    Abcam's Catalase Assay Kit provides a highly sensitive, simple, direct and HTS-ready assay for measuring catalase activity in biological samples. In the assay, catalase first reacts with H2O2 to produce water and oxygen, the unconverted H2O2 reacts with OxiRed probe to produce a product, which can be measured at 570 nm (Colorimetric method) or at Ex/Em=535/587 nm (fluorometric method). Catalase activity is reversely proportional to the signal. The kit can detect 1 μU of catalase activity in samples.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Catalase (EC is a ubiquitous antioxidant enzyme that is present in nearly all living organisms. It functions to catalyze the decomposition of hydrogen peroxide (H2O2) to water and oxygen.

  • Tested applicationsSuitable for: Functional Studiesmore details


  • FunctionOccurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
  • Involvement in diseaseDefects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
  • Sequence similaritiesBelongs to the catalase family.
  • Post-translational
    The N-terminus is blocked.
  • Cellular localizationPeroxisome.
  • Information by UniProt
  • Alternative names
    • CAT
    • Catalase
    • EC
    • MGC138422
    • MGC138424
    see all

Associated products


Our Abpromise guarantee covers the use of ab83464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Catalase Assay Kit images

  • H2O2 standard curve using ab83464.
  • Sample tests using ab83464.


References for Catalase Assay Kit (ab83464)

This product has been referenced in:
  • Kim J  et al. SOD3 acts as a tumor suppressor in PC-3 prostate cancer cells via hydrogen peroxide accumulation. Anticancer Res 34:2821-31 (2014). Read more (PubMed: 24922645) »
  • Salcher S  et al. C10ORF10/DEPP, a transcriptional target of FOXO3, regulates ROS-sensitivity in human neuroblastoma. Mol Cancer 13:224 (2014). Read more (PubMed: 25261981) »

See all 3 Publications for this product

Product Wall

We have added the stop solution for ab83464 to our catalog as ab176555. A link to the product datasheet is given below:


The High Control (HC) is a well that has the same volume of sample (2-78 uL) loaded as your sample wells. If you load wells with different volumes of sample, you will need to also load multiple HC wells with the same volume of sample. Prior to add...

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Gracias por contactarnos.

En el kit tiene lugar la reacción de H2O2 para producir H2O y oxigeno catalizado por la enzima catalasa. El H2O2 que queda sin reaccionar reacciona con OxiRed™ generando un producto que presenta absorban...

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Thank you for contacting us.

I can confirm that the fluorimetric detection method can be used with the 545/590 filter. Please note that the expected results could be of less quality as reading results with a 535/587 filter would be.


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Thank you for contacting us. I am sorry to hear that you have had problems with our Catalase Assay Kit (ab83464)

I would like to reassure you that this kit is tested and covered by our 6 month guarantee for Tissue extracts. In the event that ...

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Thank you very much for your interest in our products.

To our knowledge, we have do not have a Catalase Assay kit in our catalog that is tested and guaranteed for plants. Since ab83464 is working by measuring the the unconverted H2O2, w...

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Thank you for your reply.

As for your samples, the dilution is extremely important and from your previous results it seems that 1:20 is best of the three you have already tested. Perhaps testing 1:25 and/or 1:30 would also be useful to ch...

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Thank you for your patience as I have investigated this issue further with the laboratory.

I can confirm that you performed the assay correctly the second time, only adding stop solution to the standards and HC ONCE.

It is n...

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The HC and standard wells already contain the stop solution. You need NOT add any more stop solution to these wells. Add the stop solution only to the sample wells in step 3 of this protocol.

Hope this information has been helpful for y...

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Thank you for your reply.

Just to confirm with the stop solution - did you add this to the HC samples as in step 1C? How did you perform the assay for the data you sent me previously (did you add stop solution to your HC once or twice)? C...

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