Anti-Cathelicidin/CLP antibody (ab180760)
Key features and details
- Rabbit polyclonal to Cathelicidin/CLP
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Cathelicidin/CLP antibody
See all Cathelicidin/CLP primary antibodies -
Description
Rabbit polyclonal to Cathelicidin/CLP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Macaque monkey, Rhesus monkey, Gorilla, African green monkey, Orangutan -
Immunogen
Recombinant full length protein corresponding to Human Cathelicidin/CLP aa 31-170.
Sequence:QVLSYKEAVL RAIDGINQRS SDANLYRLLD LDPRPTMDGD PDTPKPVSFT VKETVCPRTT QQSPEDCDFK KDGLVKRCMG TVTLNQARGS FDISCDKDNK RFALLGDFFR KSKEKIGKEF KRIVQRIKDF LRNLVPRTES
Database link: P49913 -
Positive control
- WB: MCF7, SGC-7901, A-549 cell lysates, mouse testis tissue lysate; IHC-P: mouse testis, mouse spleen, human lung, human tonsil tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 19 kDa.
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IHC-P |
1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 19 kDa. |
IHC-P
1/50 - 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity. -
Tissue specificity
Expressed in bone marrow and testis and neutrophils. -
Sequence similarities
Belongs to the cathelicidin family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 741881 Chimpanzee
- Entrez Gene: 820 Human
- Entrez Gene: 12796 Mouse
- Entrez Gene: 619186 Rhesus monkey
- Omim: 600474 Human
- SwissProt: Q1KLY6 African green monkey
- SwissProt: Q1KLX1 Chimpanzee
- SwissProt: Q1KLX7 Cynomolgus monkey
see all -
Alternative names
- 18 kDa cationic antimicrobial protein antibody
- Antibacterial peptide LL-37 antibody
- Antibacterial protein FALL-39 antibody
see all
Images
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Paraffin-embedded mouse testis tissue stained for Cathelicidin/CLP using ab180760 at 1/200 dilution in immunohistochemical analysis.
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All lanes : Anti-Cathelicidin/CLP antibody (ab180760) at 1/1000 dilution
Lane 1 : MCF7 cell extract
Lane 2 : SGC-7901 cell extract
Lane 3 : A-549 cell extract
Lane 4 : Mouse testis tissue extract
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG
Developed using the ECL technique.
Predicted band size: 19 kDa
Exposure time: 60 seconds -
Paraffin-embedded human tonsil tissue stained for Cathelicidin/CLP using ab180760 at 1/200 dilution in immunohistochemical analysis.
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Paraffin-embedded mouse spleen tissue stained for Cathelicidin/CLP using ab180760 at 1/200 dilution in immunohistochemical analysis.
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Paraffin-embedded human lung tissue stained for Cathelicidin/CLP using ab180760 at 1/200 dilution in immunohistochemical analysis.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab180760 has been referenced in 7 publications.
- Johnson V et al. Activated Mesenchymal Stromal Cell Therapy for Treatment of Multi-Drug Resistant Bacterial Infections in Dogs. Front Vet Sci 9:925701 (2022). PubMed: 35812842
- Liu Z et al. Paeoniflorin inhibits the macrophage-related rosacea-like inflammatory reaction through the suppressor of cytokine signaling 3-apoptosis signal-regulating kinase 1-p38 pathway. Medicine (Baltimore) 100:e23986 (2021). PubMed: 33545988
- Chow L et al. Antibacterial activity of human mesenchymal stem cells mediated directly by constitutively secreted factors and indirectly by activation of innate immune effector cells. Stem Cells Transl Med 9:235-249 (2020). PubMed: 31702119
- Zhang K et al. Sodium Butyrate Abrogates the Growth and Pathogenesis of Mycobacterium bovis via Regulation of Cathelicidin (LL37) Expression and NF-?B Signaling. Front Microbiol 11:433 (2020). PubMed: 32265874
- Kumagai Y et al. Antimicrobial peptide LL-37 ameliorates a murine sepsis model via the induction of microvesicle release from neutrophils. Innate Immun 26:565-579 (2020). PubMed: 32600088
- Yang X et al. LL-37-Induced Autophagy Contributed to the Elimination of Live Porphyromonas gingivalis Internalized in Keratinocytes. Front Cell Infect Microbiol 10:561761 (2020). PubMed: 33178622
- Wang J et al. Allergen specific immunotherapy enhanced defense against bacteria via TGF-ß1-induced CYP27B1 in asthma. Oncotarget 8:68681-68695 (2017). IHC-Fr, WB ; Mouse, Human . PubMed: 28978148