Overview

  • Product nameAnti-Cathepsin B antibody
    See all Cathepsin B primary antibodies
  • Description
    Rabbit polyclonal to Cathepsin B
  • Specificityab30443 recognises liver Cathepsin B. Does not crossreact with Cathepsin L or Cathepsin H.
  • Tested applicationsSuitable for: ELISA, WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified): Cathepsin B from Human liver.

Properties

Applications

Our Abpromise guarantee covers the use of ab30443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/250 - 1/500.
WB 1/1000 - 1/5000. Predicted molecular weight: 38 kDa.
ICC/IF Use at an assay dependent concentration. See Abreview.
IP Use at an assay dependent concentration. PubMed: 24833013

Target

  • FunctionThiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
  • Sequence similaritiesBelongs to the peptidase C1 family.
  • Cellular localizationLysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amyloid precursor protein secretase antibody
    • APP secretase antibody
    • APPS antibody
    • CATB_HUMAN antibody
    • Cathepsin B heavy chain antibody
    • Cathepsin B1 antibody
    • CathepsinB antibody
    • CPSB antibody
    • CTSB antibody
    • cysteine protease antibody
    • OTTHUMP00000116009 antibody
    • OTTHUMP00000229510 antibody
    • OTTHUMP00000229511 antibody
    • OTTHUMP00000229512 antibody
    • OTTHUMP00000229514 antibody
    • OTTHUMP00000229515 antibody
    • OTTHUMP00000229516 antibody
    • Preprocathepsin B antibody
    see all

References for Anti-Cathepsin B antibody (ab30443)

This product has been referenced in:
  • Lohoefer F  et al. Quantitative expression and localization of cysteine and aspartic proteases in human abdominal aortic aneurysms. Exp Mol Med 46:e95 (2014). WB, IP ; Human . Read more (PubMed: 24833013) »
  • Krohne TU  et al. Lipid peroxidation products reduce lysosomal protease activities in human retinal pigment epithelial cells via two different mechanisms of action. Exp Eye Res 90:261-6 (2010). Read more (PubMed: 19895809) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (pancreatic cancer cell line)
Specification pancreatic cancer cell line
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% triton X
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
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Submitted Jul 18 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"