Western blot - Anti-Cathepsin B antibody [CA10] (ab58802)
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 38 kDa
Exposure time : 10 seconds
Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [CA10] (ab58802)Image from Shimizu A et al., The Journal of Biological Chemistry, 288, 2210-2222, January 25, 2013. Fig 5.; doi: 10.1074/jbc.M112.397398
Immunofluorescence analysis of U87MG cells incubated with netrin-1, staining Cathepsin B with ab58802.
Flow Cytometry - Anti-Cathepsin B antibody [CA10] (ab58802)
Overlay histogram showing HepG2 cells stained with ab58802 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58802, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [CA10] (ab58802)Image courtesy of Dr Irina Teplova by Abreview.
ab58802 staining Cathepsin B in murine mammary gland tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Samples were then blocked and then incubated with ab58802 at a 1/200 dilution for 30 minutes at 22°C. The secondary used was a biotin conjugated rabbit anti-mouse monoclonal at a 1/250 dilution.
Fernandez-Fernandez MR et al. 3D electron tomography of brain tissue unveils distinct Golgi structures that sequester cytoplasmic contents in neurons. J Cell Sci130:83-89 (2017).
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