Anti-Cathepsin B antibody [EPR4323] (ab125067)


  • Product name
    Anti-Cathepsin B antibody [EPR4323]
    See all Cathepsin B primary antibodies
  • Description
    Rabbit monoclonal [EPR4323] to Cathepsin B
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Cathepsin B aa 1-200. The exact sequence is proprietary.

  • Positive control
    • Human kidney and papillary carcinoma tissues; HeLa cells; HT1080, A375, SW480 and A549 cell line lysates.
  • General notes

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab125067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
    • Sequence similarities
      Belongs to the peptidase C1 family.
    • Cellular localization
      Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
    • Alternative names
      • Amyloid precursor protein secretase antibody
      • APP secretase antibody
      • APPS antibody
      • CATB_HUMAN antibody
      • Cathepsin B heavy chain antibody
      • Cathepsin B1 antibody
      • CathepsinB antibody
      • CPSB antibody
      • CTSB antibody
      • cysteine protease antibody
      • OTTHUMP00000116009 antibody
      • OTTHUMP00000229510 antibody
      • OTTHUMP00000229511 antibody
      • OTTHUMP00000229512 antibody
      • OTTHUMP00000229514 antibody
      • OTTHUMP00000229515 antibody
      • OTTHUMP00000229516 antibody
      • Preprocathepsin B antibody
      see all

    Anti-Cathepsin B antibody [EPR4323] images

    • ab125067, at a 1/50 dilution, staining Cathepsin B in paraffin-embedded Human kidney tissue by immunohistochemistry.
    • ab125067, at a 1/50 dilution, staining Cathepsin B (red) in HeLa cells by immunofluorescence. DNA is marked in blue.
    • All lanes : Anti-Cathepsin B antibody [EPR4323] (ab125067) at 1/1000 dilution

      Lane 1 : HT1080 cell lysate
      Lane 2 : A375 cell lysate
      Lane 3 : SW480 cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Goat anti-Rabbit HRP at 1/2000 dilution
      Developed using the ECL technique

      Predicted band size : 38 kDa
    • ab125067, at a 1/50 dilution, staining Cathepsin B in paraffin-embedded Human papillary carcinoma tissue by immunohistochemistry.

    References for Anti-Cathepsin B antibody [EPR4323] (ab125067)

    ab125067 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us and your interest in our products.
    Please find the ICC protocol used with the Anti-Cathepsin B antibody [EPR4323] (ab125067) attached to this email.
    The secondary antibody used in the staining was an Alexa Fluor 56...

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