Overview

  • Product nameAnti-Cathepsin D antibody
    See all Cathepsin D primary antibodies
  • Description
    Rabbit polyclonal to Cathepsin D
  • Specificityab49787 recognizes the light chain of cleaved cathepsin D, as well as the intact zymogen.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide based on the N terminus of the light chain of human cathepsin D

Properties

Applications

Our Abpromise guarantee covers the use of ab49787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 46 kDa. The apparent mass may be increased due to glycosylation. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionAcid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
    • Tissue specificityExpressed in the aorta extrcellular space (at protein level).
    • Involvement in diseaseCeroid lipofuscinosis, neuronal, 10
    • Sequence similaritiesBelongs to the peptidase A1 family.
      Contains 1 peptidase A1 domain.
    • Post-translational
      modifications
      N- and O-glycosylated.
    • Cellular localizationLysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
    • Information by UniProt
    • Database links
    • Alternative names
      • CatD antibody
      • CATD_HUMAN antibody
      • Cathepsin D heavy chain antibody
      • CD antibody
      • Ceroid lipofuscinosis neuronal 10 antibody
      • CLN10 antibody
      • CPSD antibody
      • ctsd antibody
      • Epididymis secretory sperm binding protein Li 130P antibody
      • HEL S 130P antibody
      • Lysosomal aspartyl peptidase antibody
      • Lysosomal aspartyl protease antibody
      • MGC2311 antibody
      see all

    Anti-Cathepsin D antibody images

    • All lanes : Anti-Cathepsin D antibody (ab49787) at 1/1000 dilution

      Lane 1 : Recombinant Human Cathepsin D at 0.04 µg
      Lane 2 : Recombinant Human Cathepsin D at 0.02 µg
      Lane 3 : Recombinant Human Cathepsin D at 0.01 µg
      Lane 4 : Recombinant Human Cathepsin D at 0.004 µg
      Lane 5 : Recombinant Human Cathepsin D at 0.002 µg


      Predicted band size : 46 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)

    References for Anti-Cathepsin D antibody (ab49787)

    This product has been referenced in:
    • Harrell JC  et al. Estrogen insensitivity in a model of estrogen receptor positive breast cancer lymph node metastasis. Cancer Res 67:10582-91 (2007). Read more (PubMed: 17975003) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"