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Cathepsin D isolated from human liver
Our Abpromise guarantee covers the use of ab826 in the following tested applications.
|IHC-P||1/75 - 1/100. ABC method.|
|IHC-Fr||1/75 - 1/100. ABC method.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 19505575|
|ICC/IF||Use a concentration of 10 µg/ml.|
ICC/IF image of ab826 stained A431 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab826, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.