Overview

  • Product nameAnti-Cathepsin D antibody
    See all Cathepsin D primary antibodies
  • Description
    Rabbit polyclonal to Cathepsin D
  • SpecificityThis antibody reacts with the zymogen as well as the activated form of cathepsin D. It stains fibroblasts and macrophages strongly but stains weakly with the myoepithelial cells of breast. It also stains sweat ducts and glands in the skin, islets cells and granules of pancreatic acini.
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, IHC-FoFr, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Cathepsin D isolated from human liver

  • Positive control
    • ICC/IF: A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/75 - 1/100. ABC method.
IHC-Fr 1/75 - 1/100. ABC method.
IHC-FoFr Use at an assay dependent concentration. PubMed: 19505575
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionAcid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
  • Tissue specificityExpressed in the aorta extrcellular space (at protein level).
  • Involvement in diseaseCeroid lipofuscinosis, neuronal, 10
  • Sequence similaritiesBelongs to the peptidase A1 family.
    Contains 1 peptidase A1 domain.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localizationLysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
  • Information by UniProt
  • Database links
  • Alternative names
    • CatD antibody
    • CATD_HUMAN antibody
    • Cathepsin D heavy chain antibody
    • CD antibody
    • Ceroid lipofuscinosis neuronal 10 antibody
    • CLN10 antibody
    • CPSD antibody
    • ctsd antibody
    • Epididymis secretory sperm binding protein Li 130P antibody
    • HEL S 130P antibody
    • Lysosomal aspartyl peptidase antibody
    • Lysosomal aspartyl protease antibody
    • MGC2311 antibody
    see all

Anti-Cathepsin D antibody images

  • ICC/IF image of ab826 stained A431 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab826, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab826 staining Cathepsin D in formalin-fixed, paraffin-embedded Human prostate tissue by Immunohistochemistry.
  • Immunohistochemical analysis of PFA-perfusion fixed, frozen rat brain tissue sections (substantia nigra), staining Cathepsin D with ab826. Samples were incubated with primary antibody overnight at room temperature.

References for Anti-Cathepsin D antibody (ab826)

This product has been referenced in:
  • Chu Y  et al. Alterations in lysosomal and proteasomal markers in Parkinson's disease: relationship to alpha-synuclein inclusions. Neurobiol Dis 35:385-98 (2009). IHC-FoFr ; Rat . Read more (PubMed: 19505575) »
  • Merseburger AS  et al. Cathepsin D expression in renal cell cancer-clinical implications. Eur Urol 48:519-26 (2005). IHC-P ; Human . Read more (PubMed: 16115525) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Rat Cell lysate - whole cell (brain)
Specification brain
Blocking step Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Oct 27 2014

Es freut mich, dass ich Ihnen helfen konnte.

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Ihre neuen Ergebnisse lassen darauf schließen, dass dieser Antikörper für Ratte funktioniert und scheinbar weiterer Optimierung bedarf. Da dieser Antikö...

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Vielen Dank für dieses Update.

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Vielen Dank für Ihren Anruf und dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"