Overview

  • Product nameAnti-Cathepsin D antibody [CTD-19]
    See all Cathepsin D primary antibodies
  • Description
    Mouse monoclonal [CTD-19] to Cathepsin D
  • SpecificityRecognizes cathepsin D (34 kDa and weaker band at 52 kDa) in immunoblotting. Does not react with bovine cathepsin D and B, or with human cathepsin types B, C, G or H.
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length native protein (purified) (Human liver).

  • Positive control
    • Human breast carcinoma tissue or cell line extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab6313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 52 kDa.
ELISA Use at an assay dependent concentration.
IHC-P 1/200.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration. PubMed: 24833013

Target

  • FunctionAcid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
  • Tissue specificityExpressed in the aorta extrcellular space (at protein level).
  • Involvement in diseaseCeroid lipofuscinosis, neuronal, 10
  • Sequence similaritiesBelongs to the peptidase A1 family.
    Contains 1 peptidase A1 domain.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localizationLysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
  • Information by UniProt
  • Database links
  • Alternative names
    • CatD antibody
    • CATD_HUMAN antibody
    • Cathepsin D antibody
    • Cathepsin D heavy chain antibody
    • CD antibody
    • Ceroid lipofuscinosis neuronal 10 antibody
    • CLN10 antibody
    • CPSD antibody
    • ctsd antibody
    • Epididymis secretory sperm binding protein Li 130P antibody
    • HEL S 130P antibody
    • Lysosomal aspartyl peptidase antibody
    • Lysosomal aspartyl protease antibody
    • MGC2311 antibody
    see all

Anti-Cathepsin D antibody [CTD-19] images

  • ICC/IF image of ab6313 stained human HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6313, 5 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, Hek293 and MCF7 cells.

  • Anti-Cathepsin D antibody [CTD-19] (ab6313) at 1/4000 dilution + MCF7 Human breast carcinoma cells

References for Anti-Cathepsin D antibody [CTD-19] (ab6313)

This product has been referenced in:
  • Tohnai G  et al. Paeoniflorin eliminates a mutant AR via NF-YA-dependent proteolysis in spinal and bulbar muscular atrophy. Hum Mol Genet N/A:N/A (2014). Mouse . Read more (PubMed: 24549037) »
  • Lohoefer F  et al. Quantitative expression and localization of cysteine and aspartic proteases in human abdominal aortic aneurysms. Exp Mol Med 46:e95 (2014). WB, IP ; Human . Read more (PubMed: 24833013) »

See all 13 Publications for this product

Product Wall

Application Western blot
Sample Human Tissue lysate - whole (CSF)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 50 µg
Specification CSF
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 11 2015

Application Western blot
Sample Mouse Tissue lysate - whole (brain tissue)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 50 µg
Specification brain tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 11 2015

Application Western blot
Loading amount 30 µg
Gel Running Conditions Non-reduced Denaturing (10)
Sample Human Cell lysate - other (Glioblastoma cell lines)
Specification Glioblastoma cell lines
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Mr. Achilleas Mitrakas

Verified customer

Submitted Apr 25 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (DU145 Human prostate)
Specification DU145 Human prostate
Permeabilization Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

Submitted Nov 21 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (adipocyte tissue)
Loading amount 35 µg
Specification adipocyte tissue
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 18 2012

Vielen Dank für Ihre Anfrage die Herr Hryca uns in den technischen Dienst weitergeleitet hat.

Ich habe geschaut, und wir haben viele möglichen positiven Kontrollen für Sie. Können Sie mir bitte noch sagen, welche Art von p...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HepaRG)
Specification HepaRG
Fixative Formaldehyde
Permeabilization Yes - Triton X-100 in PBS
Blocking step Milk as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mrs. Alina Macovei

Verified customer

Submitted May 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HepaRG)
Loading amount 10 µg
Specification HepaRG
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Mrs. Alina Macovei

Verified customer

Submitted May 03 2012

1-10 of 23 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"