Anti-Cathepsin D antibody [CTD-19] (ab6313)
Key features and details
- Mouse monoclonal [CTD-19] to Cathepsin D
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Cathepsin D antibody [CTD-19]
See all Cathepsin D primary antibodies -
Description
Mouse monoclonal [CTD-19] to Cathepsin D -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human Cathepsin D.
Database link: P07339 -
Positive control
- Human breast carcinoma tissue or cell line extract.
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General notes
This product was changed from ascites to tissue culture supernatant on 21 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.097% Sodium azide -
Concentration information loading...
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Purity
Proprietary Purification -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
CTD-19 -
Isotype
IgG2a -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6313 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (12) |
Use at an assay dependent concentration. Detects a band of approximately 52 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 52 kDa. |
Target
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Function
Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. -
Tissue specificity
Expressed in the aorta extrcellular space (at protein level). -
Involvement in disease
Ceroid lipofuscinosis, neuronal, 10 -
Sequence similarities
Belongs to the peptidase A1 family.
Contains 1 peptidase A1 domain. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380). - Information by UniProt
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Database links
- Entrez Gene: 1509 Human
- Omim: 116840 Human
- SwissProt: P07339 Human
- Unigene: 654447 Human
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Alternative names
- CatD antibody
- CATD_HUMAN antibody
- Cathepsin D antibody
see all
Images
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Anti-Cathepsin D antibody [CTD-19] (ab6313) at 1/1000 dilution + MDA MB 231 whole cell lysate at 20 µg
Secondary
HRP-conjugated monoclonal sheep anti-mouse IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 28,48,50 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesBlocked with 5% milk for 1 hour at 22°C.
Incubated with the primary antibody for 1 hour at 22°C in 5% TBS-T.
This image was generated using the ascites version of the product.
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Anti-Cathepsin D antibody [CTD-19] (ab6313) at 1/4000 dilution + MCF7 Human breast carcinoma cells
This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (64)
ab6313 has been referenced in 64 publications.
- Cao SQ et al. P. edulis Extract Protects Against Amyloid-β Toxicity in Alzheimer's Disease Models Through Maintenance of Mitochondrial Homeostasis via the FOXO3/DAF-16 Pathway. Mol Neurobiol 59:5612-5629 (2022). PubMed: 35739408
- Chuaypen N et al. Targeted Proteins Reveal Cathepsin D as a Novel Biomarker in Differentiating Hepatocellular Carcinoma from Cirrhosis and Other Liver Cancers. Asian Pac J Cancer Prev 23:2017-2025 (2022). PubMed: 35763644
- Lucchino V et al. Insights into the Genetic Profile of Two Siblings Affected by Unverricht-Lundborg Disease Using Patient-Derived hiPSCs. Cells 11:N/A (2022). PubMed: 36359887
- Kim S et al. Endosomal mTORC2 Is Required for Phosphoinositide-Dependent AKT Activation in Platelet-Derived Growth Factor-Stimulated Glioma Cells. Cancers (Basel) 13:N/A (2021). PubMed: 34065746
- Heck AL et al. Selective HSP90β inhibition results in TNF and TRAIL mediated HIF1α degradation. Immunobiology 226:152070 (2021). PubMed: 33639524