Overview

  • Product name
    Anti-Cathepsin D antibody [CTD-19]
    See all Cathepsin D primary antibodies
  • Description
    Mouse monoclonal [CTD-19] to Cathepsin D
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length native protein (purified) (Human liver).

  • Positive control
    • Human breast carcinoma tissue or cell line extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab6313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 52 kDa.
ELISA Use at an assay dependent concentration.
IHC-P 1/200.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration. PubMed: 24833013

Target

  • Function
    Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
  • Tissue specificity
    Expressed in the aorta extrcellular space (at protein level).
  • Involvement in disease
    Ceroid lipofuscinosis, neuronal, 10
  • Sequence similarities
    Belongs to the peptidase A1 family.
    Contains 1 peptidase A1 domain.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localization
    Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
  • Information by UniProt
  • Database links
  • Alternative names
    • CatD antibody
    • CATD_HUMAN antibody
    • Cathepsin D antibody
    • Cathepsin D heavy chain antibody
    • CD antibody
    • Ceroid lipofuscinosis neuronal 10 antibody
    • CLN10 antibody
    • CPSD antibody
    • ctsd antibody
    • Epididymis secretory sperm binding protein Li 130P antibody
    • HEL S 130P antibody
    • Lysosomal aspartyl peptidase antibody
    • Lysosomal aspartyl protease antibody
    • MGC2311 antibody
    see all

Images

  • Anti-Cathepsin D antibody [CTD-19] (ab6313) at 1/1000 dilution + MDA MB 231 whole cell lysate at 20 µg

    Secondary
    HRP-conjugated monoclonal sheep anti-mouse IgG at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 28,48,50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    This image is courtesy of an anonymous Abreview

    Blocked with 5% milk for 1 hour at 22°C.

    Incubated with the primary antibody for 1 hour at 22°C in 5% TBS-T.

    See Abreview

  • ab6313 staining Cathepsin D in DU145 human prostate cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 pH 7.4 and blocked with 5% BSA at room temperature for 20 minutes. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour. A CF488A-conjugated goat anti-mouse IgG (H+L) polyclonal was used as the secondary antibody (1/500).

    See Abreview

  • ab6313 staining Cathepsin D in HepaRG cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X-100 in PBS and blocked with 1% milk at room temperature for 20 minutes. Samples were incubated with primary antibody (1µg/ml in 1% milk) for 30 minutes. An undiluted Alexa Fluor® 594-conjugated goat anti-mouse IgG  polyclonal was used as the secondary antibody.

    See Abreview

  • Anti-Cathepsin D antibody [CTD-19] (ab6313) at 1/4000 dilution + MCF7 Human breast carcinoma cells

References

This product has been referenced in:
  • Ugun-Klusek A  et al. Continued 26S proteasome dysfunction in mouse brain cortical neurons impairs autophagy and the Keap1-Nrf2 oxidative defence pathway. Cell Death Dis 8:e2531 (2017). WB ; Mouse . Read more (PubMed: 28055010) »
  • Piras A  et al. Autophagic and lysosomal defects in human tauopathies: analysis of post-mortem brain from patients with familial Alzheimer disease, corticobasal degeneration and progressive supranuclear palsy. Acta Neuropathol Commun 4:22 (2016). Read more (PubMed: 26936765) »

See all 27 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

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Application
Western blot
Sample
Human Tissue lysate - whole (CSF)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
CSF
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 11 2015

Application
Western blot
Sample
Mouse Tissue lysate - whole (brain tissue)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
brain tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 11 2015

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing (10)
Sample
Human Cell lysate - other (Glioblastoma cell lines)
Specification
Glioblastoma cell lines
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Mr. Achilleas Mitrakas

Verified customer

Submitted Apr 25 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (DU145 Human prostate)
Specification
DU145 Human prostate
Permeabilization
Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative
Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

Submitted Nov 21 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (adipocyte tissue)
Loading amount
35 µg
Specification
adipocyte tissue
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HepaRG)
Specification
HepaRG
Fixative
Formaldehyde
Permeabilization
Yes - Triton X-100 in PBS
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mrs. Alina Macovei

Verified customer

Submitted May 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HepaRG)
Loading amount
10 µg
Specification
HepaRG
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Mrs. Alina Macovei

Verified customer

Submitted May 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (breast cancer cells)
Specification
breast cancer cells
Treatment
17-beta oestradiol for 24hrs
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Manikandan Periyasamy

Verified customer

Submitted Aug 18 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MDA MB 231 cells)
Loading amount
20 µg
Specification
MDA MB 231 cells
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted May 29 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH 3T3 cells)
Loading amount
20 µg
Specification
NIH 3T3 cells
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted May 29 2009

1-10 of 12 Abreviews

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