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Full length native protein (purified) (Human liver).
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab75852 as a replacement.
Our Abpromise guarantee covers the use of ab6313 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 52 kDa.|
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration. PubMed: 24833013|
Blocked with 5% milk for 1 hour at 22°C.
Incubated with the primary antibody for 1 hour at 22°C in 5% TBS-T.
ab6313 staining Cathepsin D in DU145 human prostate cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 pH 7.4 and blocked with 5% BSA at room temperature for 20 minutes. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour. A CF488A-conjugated goat anti-mouse IgG (H+L) polyclonal was used as the secondary antibody (1/500).
ab6313 staining Cathepsin D in HepaRG cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X-100 in PBS and blocked with 1% milk at room temperature for 20 minutes. Samples were incubated with primary antibody (1µg/ml in 1% milk) for 30 minutes. An undiluted Alexa Fluor® 594-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"