The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: 1/100 - 1/250.
IHC-P: 1/500. Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.
WB: 1/1000 - 1/2000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
Is unsuitable for Flow Cyt or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
Expressed in the aorta extrcellular space (at protein level).
Involvement in disease
Ceroid lipofuscinosis, neuronal, 10
Belongs to the peptidase A1 family. Contains 1 peptidase A1 domain.
N- and O-glycosylated.
Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).