Recombinant
RabMAb

Anti-Cathepsin D antibody [EPR3056Y] (ab75811)

Overview

  • Product name
    Anti-Cathepsin D antibody [EPR3056Y]
    See all Cathepsin D primary antibodies
  • Description
    Rabbit monoclonal [EPR3056Y] to Cathepsin D
  • Tested applications
    Suitable for: Flow Cyt, WB, IHC-P, ICCmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N-terminus of human Cathepsin D

  • Positive control
    • MCF-7 and SKBR3 cell lysates. Human liver tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab75811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
WB
IHC-P
ICC
  • Application notes
    ICC: 1/100 - 1/250.
    IHC-P: 1/500. Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.
    WB: 1/1000 - 1/2000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).

    Is unsuitable for Flow Cyt or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
    • Tissue specificity
      Expressed in the aorta extrcellular space (at protein level).
    • Involvement in disease
      Ceroid lipofuscinosis, neuronal, 10
    • Sequence similarities
      Belongs to the peptidase A1 family.
      Contains 1 peptidase A1 domain.
    • Post-translational
      modifications
      N- and O-glycosylated.
    • Cellular localization
      Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
    • Information by UniProt
    • Database links
    • Alternative names
      • CatD antibody
      • CATD_HUMAN antibody
      • Cathepsin D antibody
      • Cathepsin D heavy chain antibody
      • CD antibody
      • Ceroid lipofuscinosis neuronal 10 antibody
      • CLN10 antibody
      • CPSD antibody
      • ctsd antibody
      • Epididymis secretory sperm binding protein Li 130P antibody
      • HEL S 130P antibody
      • Lysosomal aspartyl peptidase antibody
      • Lysosomal aspartyl protease antibody
      • MGC2311 antibody
      see all

    Anti-Cathepsin D antibody [EPR3056Y] images

    • All lanes : Anti-Cathepsin D antibody [EPR3056Y] (ab75811) at 1/1000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : SKBR3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 46 kDa
      Observed band size : 15,46 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded human liver using ab75811 at 1/500 dilution.

    References for Anti-Cathepsin D antibody [EPR3056Y] (ab75811)

    ab75811 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (small intestine, ganglion cells)
    Specification
    small intestine, ganglion cells
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate, 100C 20 minutes
    Permeabilization
    No
    Username

    Abcam user community

    Verified customer

    Submitted Oct 22 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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