Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1:32,000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 53 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
    • Tissue specificity
      High expression levels in heart, skeletal muscle, brain, testis and ovary; moderate levels in prostate, placenta, liver and colon; and no detectable expression in peripheral leukocytes and thymus.
    • Sequence similarities
      Belongs to the peptidase C1 family.
    • Cellular localization
      Lysosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • AI481912 antibody
      • CATF_HUMAN antibody
      • Cathepsin F antibody
      • CathepsinF antibody
      • CATSF antibody
      • CLN13 antibody
      • Ctsf antibody
      • EC 3.4.22.41 antibody
      see all

    Images

    • Anti-Cathepsin F antibody (ab36161) at 0.3 µg/ml + Human Ovary lysate (35µg protein in RIPA buffer).

      Predicted band size : 53 kDa

    References

    This product has been referenced in:
    • Jeric B  et al. N-terminally truncated forms of human cathepsin F accumulate in aggresome-like inclusions. Biochim Biophys Acta 1833:2254-66 (2013). Human . Read more (PubMed: 23684953) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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