Overview

  • Product name
    Cathepsin G Activity Assay Kit (Colorimetric)
    See all Cathepsin G kits
  • Sample type
    Serum, Tissue Extracts, Cell Lysate
  • Assay type
    Quantitative
  • Range
    0 nmol/well - 50 nmol/well
  • Assay time
    1h 00m
  • Species reactivity
    Reacts with: Mammal
  • Product overview

    Cathepsin G Activity Assay Kit (Colorimetric) (ab126780) is an assay where Cathepsin G will cleave the substrate and release the dye group, p-NA (4-Nitroaniline), which can be detected at 405 nm. In presence of the Cathepsin G specific inhibitor, the cleavage will be stopped. The kit provides a rapid, simple, sensitive, and reliable test for the activity of Cathepsin G.


    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Cathepsin G is an enzymatic protein belonging to the peptidase or protease families. The protein is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab126780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Images

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • Cathepsin G activity in THP-1 lysates (duplicates +/- SD). Data corresponds to 1 million cells per well.

  • p-NA Standard Curve. Performed according to assay protocol.

Protocols

References

This product has been referenced in:
  • Featherston T  et al. Expression and Localization of Cathepsins B, D, and G in Two Cancer Stem Cell Subpopulations in Moderately Differentiated Oral Tongue Squamous Cell Carcinoma. Front Med (Lausanne) 4:100 (2017). Read more (PubMed: 28775982) »
  • Bou Ghanem EN  et al. The Alpha-Tocopherol Form of Vitamin E Boosts Elastase Activity of Human PMNs and Their Ability to Kill Streptococcus pneumoniae. Front Cell Infect Microbiol 7:161 (2017). Functional Studies . Read more (PubMed: 28516066) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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