Cathepsin G Activity Assay Kit (Colorimetric) (ab126780) is an assay where Cathepsin G will cleave the substrate and release the dye group, p-NA (4-Nitroaniline), which can be detected at 405 nm. In presence of the Cathepsin G specific inhibitor, the cleavage will be stopped. The kit provides a rapid, simple, sensitive, and reliable test for the activity of Cathepsin G.
Cathepsin G is an enzymatic protein belonging to the peptidase or protease families. The protein is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation.
FunctionSerine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe-CH2Cl and phenylmethylsulfonyl fluoride.
Sequence similaritiesBelongs to the peptidase S1 family. Contains 1 peptidase S1 domain.