Cathepsin G Inhibitor Screening Assay Kit (Colorimetric) (ab204693)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Inhibitor compounds
Overview
-
Product name
Cathepsin G Inhibitor Screening Assay Kit (Colorimetric)
See all Cathepsin G kits -
Detection method
Colorimetric -
Sample type
Inhibitor compounds -
Assay type
Enzyme activity -
Product overview
Cathepsin G Inhibitor Screening Assay Kit (Colorimetric) (ab204693) uses the ability of active Cathepsin G to cleave a synthetic pNA (4-Nitroaniline)-based peptide substrate to release pNA, which can be easily quantified using a colorimetric microplate reader. In the presence of a Cathepsin G inhibitor, the cleavage of this substrate is reduced/abolished resulting in decrease or total loss of the pNA absorbance. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of Cathepsin G.
-
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K162 Cathepsin G Inhibitor Screening Kit (Colorimetric). K162-100 is the same size as the 100 test size of ab204693.
Cathepsin G (CTSG, EC 3.4.21.20) is a Serine protease found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation.
-
Platform
Microplate reader
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Cathepsin G Inhibitor 1 x 20µl Cathepsin G Substrate 1 x 200µl CTSG Assay Buffer 1 x 20ml Human Cathepsin G II 1 vial -
Research areas
-
Function
Serine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe-CH2Cl and phenylmethylsulfonyl fluoride. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain. -
Cellular localization
Cell surface. - Information by UniProt
-
Alternative names
- CATG
- CATG_HUMAN
- Cathepsin G
see all
Associated products
-
Related Products
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab204693 has been referenced in 1 publication.
- Goldberg EL et al. ß-Hydroxybutyrate Deactivates Neutrophil NLRP3 Inflammasome to Relieve Gout Flares. Cell Rep 18:2077-2087 (2017). PubMed: 28249154