Cathepsin G overexpression 293T lysate (whole cell) (ab94086)

Overview

  • Product name
    Cathepsin G overexpression 293T lysate (whole cell)
  • Description
    Cathepsin G overexpression lysate
  • General notes
    ab94086 is a 293T cell transfected lysate in which Human Cathepsin G has been transiently over-expressed using a pCMV-Cathepsin G plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details of how the transfected lysate was prepared view preparation notes
  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • Purity
    Whole Cell Lysate
  • Research areas
  • Background
    Function: Serine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe-CH2Cl and phenylmethylsulfonyl fluoride. Similarity: Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Images

  • ab94086 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Cathepsin G antibody (ab89593) at 1/500 dilution

    Lane 1 : Cathepsin G overexpression 293T lysate (whole cell) (ab94086)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References

ab94086 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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