Cathepsin H Activity Assay Kit (Fluorometric) (ab65305)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 2 hr
- Sample type: Cell Lysate, Tissue Extracts
Overview
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Product name
Cathepsin H Activity Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Tissue Extracts, Cell Lysate -
Assay type
Quantitative -
Assay time
2h 00m -
Product overview
Cathepsin H Activity Assay Kit (Fluorometric) (ab65305) is a fluorescence-based assay that utilizes the preferred cathepsin-B substrate Arginine labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-B will cleave the synthetic substrate R-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader.The released AFC can easily be quantified using a fluorometer or fluorescence plate reader. The Cathepsin H assay is simple, straightforward, and can be adapted to 96-well plate assays. Assay conditions have been optimized to obtain the maximal activity.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K145 Cathepsin H Activity Fluorometric Assay Kit. K145-100 is the same size as the 100 test size of ab65305.
Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests CH Cell Lysis Buffer 1 x 25ml CH Inhibitor (1 mM) 1 x 20µl CH Reaction Buffer 1 x 5ml CH Substrate R-AFC (10 mM) 1 x 200µl -
Research areas
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Relevance
The cathepsin family of proteolytic enzymes contains several diverse classes of proteases. The cysteine protease class comprises cathepsins B, L, H, K, S, and O. The aspartyl protease class is composed of cathepsins D and E. Cathepsin G is in the serine protease class. Most cathepsins are lysosomal and each is involved in various cellular events such as peptide biosynthesis and protein degradation. The propeptide region of cathepsin H is highly homologous to other cathepsins. This region is thought to play a role in the processing of cysteine proteases. Cathepsin H is thought to be involved in several pathological conditions, including neuro degenerative disorders and cancer. -
Cellular localization
Lysosome -
Alternative names
- ACC 4
- ACC 5
- ACC4
see all
Associated products
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Related Products
Images
Datasheets and documents
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Datasheet download
References (0)
ab65305 has not yet been referenced specifically in any publications.