Cathepsin H Activity Assay Kit (Fluorometric) (ab65305)
- Product nameCathepsin H Activity Assay Kit (Fluorometric)
- Tests100 x 1 assay
- Sample typeTissue Extracts, Cell Lysate
- Assay typeQuantitative
- Assay time2h 00m
- Product overview
Abcam's Cathepsin H Activity Assay Kit (Fluorometric) is a fluorescence-based assay that utilizes the preferred cathepsin-B substrate Arginine labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-B will cleave the synthetic substrate R-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader.The released AFC can easily be quantified using a fluorometer or fluorescence plate reader. The Cathepsin H assay is simple, straightforward, and can be adapted to 96-well plate assays. Assay conditions have been optimized to obtain the maximal activity.
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Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests CH Cell Lysis Buffer WM 1 x 25ml CH Inhibitor (1 mM) Red 1 x 20µl CH Reaction Buffer NM 1 x 5ml CH Substrate R-AFC (10 mM) Amber 1 x 200µl
- Research Areas
- ACC 4ACC 5ACC4
- ACC5AleurainCathepsin B3Cathepsin BACathepsinB3CathepsinBACathepsinHCPSBCTSHDKFZp686B24257MGC1519MinichainN benzoylarginine beta naphthylamide hydrolase
Our Abpromise guarantee covers the use of ab65305 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Cathepsin H Activity Assay Kit (Fluorometric) images
Cathepsin H assays were performed using various numbers of Jurkat cells (A) or various amounts of purified human liver cathepsin H (B), as indicated. Results were analyzed using a fluorescence plate reader (Ex/Em = 400/505 nm) as described in the kit instructions.
References for Cathepsin H Activity Assay Kit (Fluorometric) (ab65305)
ab65305 has not yet been referenced specifically in any publications.