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Cathepsin H Activity Assay Kit (Fluorometric) (ab65305)

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Overview

  • Product nameCathepsin H Activity Assay Kit (Fluorometric)
  • Tests
    100 x 1 assay
  • Sample type
    Tissue Extracts, Cell Lysate
  • Assay typeQuantitative
  • Assay time
    2h 00m
  • Product overview

    Abcam's Cathepsin H Activity Assay Kit (Fluorometric) is a fluorescence-based assay that utilizes the preferred cathepsin-B substrate Arginine labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-B will cleave the synthetic substrate R-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader.The released AFC can easily be quantified using a fluorometer or fluorescence plate reader. The Cathepsin H assay is simple, straightforward, and can be adapted to 96-well plate assays. Assay conditions have been optimized to obtain the maximal activity.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.

  • Tested applicationsFunctional Studies more details

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    CH Cell Lysis Buffer WM 1 x 25ml
    CH Inhibitor (1 mM) Red 1 x 20µl
    CH Reaction Buffer NM 1 x 5ml
    CH Substrate R-AFC (10 mM) Amber 1 x 200µl
  • Research Areas
  • RelevanceThe cathepsin family of proteolytic enzymes contains several diverse classes of proteases. The cysteine protease class comprises cathepsins B, L, H, K, S, and O. The aspartyl protease class is composed of cathepsins D and E. Cathepsin G is in the serine protease class. Most cathepsins are lysosomal and each is involved in various cellular events such as peptide biosynthesis and protein degradation. The propeptide region of cathepsin H is highly homologous to other cathepsins. This region is thought to play a role in the processing of cysteine proteases. Cathepsin H is thought to be involved in several pathological conditions, including neuro degenerative disorders and cancer.
  • Cellular localizationLysosome
    • Alternative names
        ACC 4ACC 5ACC4
        ACC5AleurainCathepsin B3Cathepsin BACathepsinB3CathepsinBACathepsinHCPSBCTSHDKFZp686B24257MGC1519MinichainN benzoylarginine beta naphthylamide hydrolase
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    Applications

    Our Abpromise guarantee covers the use of ab65305 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Cathepsin H Activity Assay Kit (Fluorometric) images

    • Functional Studies - Cathepsin H Activity Fluorometric Assay Kit (ab65305)
      Functional Studies - Cathepsin H Activity Fluorometric Assay Kit (ab65305)
      Cathepsin H assays were performed using various numbers of Jurkat cells (A) or various amounts of purified human liver cathepsin H (B), as indicated. Results were analyzed using a fluorescence plate reader (Ex/Em = 400/505 nm) as described in the kit instructions.

    Protocols

    References for Cathepsin H Activity Assay Kit (Fluorometric) (ab65305)

    ab65305 has not yet been referenced specifically in any publications.

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