Cathepsin H Activity Assay Kit (Fluorometric) (ab65305)

Overview

  • Product name
    Cathepsin H Activity Assay Kit (Fluorometric)
    See all Cathepsin H kits
  • Sample type
    Tissue Extracts, Cell Lysate
  • Assay type
    Quantitative
  • Assay time
    2h 00m
  • Product overview

    Cathepsin H Activity Assay Kit (Fluorometric) (ab65305) is a fluorescence-based assay that utilizes the preferred cathepsin-B substrate Arginine labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-B will cleave the synthetic substrate R-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader.The released AFC can easily be quantified using a fluorometer or fluorescence plate reader. The Cathepsin H assay is simple, straightforward, and can be adapted to 96-well plate assays. Assay conditions have been optimized to obtain the maximal activity.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    CH Cell Lysis Buffer WM 1 x 25ml
    CH Inhibitor (1 mM) Red 1 x 20µl
    CH Reaction Buffer NM 1 x 5ml
    CH Substrate R-AFC (10 mM) Amber 1 x 200µl
  • Research areas
  • Relevance
    The cathepsin family of proteolytic enzymes contains several diverse classes of proteases. The cysteine protease class comprises cathepsins B, L, H, K, S, and O. The aspartyl protease class is composed of cathepsins D and E. Cathepsin G is in the serine protease class. Most cathepsins are lysosomal and each is involved in various cellular events such as peptide biosynthesis and protein degradation. The propeptide region of cathepsin H is highly homologous to other cathepsins. This region is thought to play a role in the processing of cysteine proteases. Cathepsin H is thought to be involved in several pathological conditions, including neuro degenerative disorders and cancer.
  • Cellular localization
    Lysosome
  • Alternative names
    • ACC 4
    • ACC 5
    • ACC4
    • ACC5
    • Aleurain
    • Cathepsin B3
    • Cathepsin BA
    • CathepsinB3
    • CathepsinBA
    • CathepsinH
    • CPSB
    • CTSH
    • DKFZp686B24257
    • MGC1519
    • Minichain
    • N benzoylarginine beta naphthylamide hydrolase
    see all

Associated products

Applications

Our Abpromise guarantee covers the use of ab65305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Images

  • Cathepsin H assays were performed using various numbers of Jurkat cells (A) or various amounts of purified human liver cathepsin H (B), as indicated. Results were analyzed using a fluorescence plate reader (Ex/Em = 400/505 nm) as described in the kit instructions.

Protocols

References

ab65305 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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